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| Status |
Public on Jan 21, 2021 |
| Title |
RNA-sequencing of Chronic Phase Shift Protocol on Murine Pancreas |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The objective of this study was to understand the long-term effects of a chronic phase shift protocol on the pancreatic transcriptome. A total of 144 wild-type C57Bl/6J 4-6 week old mice were housed under normal circadian conditions or disrupted using a chronic phase shift protocol whereby the light-dark cycle is advanced 8 hour every 2-3 days for 4 weeks. 12 hours prior to sacrafice mice were normalized under normal circadian conditions. Starting at ZT 0, mice were serially sacraficed at 4-hour intervals for 48 hours and the pancreas extracted. Pancreatic RNA was isolated and subjected to RNA-seq.
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| Overall design |
A total of 144 samples were collected. Males (n = 3) and females (n=3) from each timepoint were pooled prior to sequencing. A total of 6 mice were sacraficed for each condition (normal circadian and circadian disrupted) at each timepoint.
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| |
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| Contributor(s) |
Schwartz PB, Berres M, Walcheck M, Ronnekleiv-Kelly SM |
| Citation(s) |
34056925, 37262074 |
| |
| Submission date |
Jan 20, 2021 |
| Last update date |
Sep 08, 2023 |
| Contact name |
Sean M Ronnekleiv-Kelly |
| E-mail(s) |
ronnekleiv-kelly@surgery.wisc.edu
|
| Organization name |
University of Wisconsin
|
| Department |
Surgery
|
| Lab |
Ronnekleiv-Kelly
|
| Street address |
1400 University Ave
|
| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (48)
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| Relations |
| BioProject |
PRJNA693534 |
| SRA |
SRP302572 |
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