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Series GSE165198 Query DataSets for GSE165198
Status Public on Jan 21, 2021
Title RNA-sequencing of Chronic Phase Shift Protocol on Murine Pancreas
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The objective of this study was to understand the long-term effects of a chronic phase shift protocol on the pancreatic transcriptome. A total of 144 wild-type C57Bl/6J 4-6 week old mice were housed under normal circadian conditions or disrupted using a chronic phase shift protocol whereby the light-dark cycle is advanced 8 hour every 2-3 days for 4 weeks. 12 hours prior to sacrafice mice were normalized under normal circadian conditions. Starting at ZT 0, mice were serially sacraficed at 4-hour intervals for 48 hours and the pancreas extracted. Pancreatic RNA was isolated and subjected to RNA-seq.
 
Overall design A total of 144 samples were collected. Males (n = 3) and females (n=3) from each timepoint were pooled prior to sequencing. A total of 6 mice were sacraficed for each condition (normal circadian and circadian disrupted) at each timepoint.
 
Contributor(s) Schwartz PB, Berres M, Walcheck M, Ronnekleiv-Kelly SM
Citation(s) 34056925
Submission date Jan 20, 2021
Last update date Sep 18, 2022
Contact name Sean M Ronnekleiv-Kelly
E-mail(s) ronnekleiv-kelly@surgery.wisc.edu
Organization name University of Wisconsin
Department Surgery
Lab Ronnekleiv-Kelly
Street address 1400 University Ave
City Madison
State/province Wisconsin
ZIP/Postal code 53706
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (48)
GSM5028088 ZT0-1
GSM5028089 ZT0-2
GSM5028090 ZT0-3
Relations
BioProject PRJNA693534
SRA SRP302572

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE165198_ZT_expression.xlsx 9.8 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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