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Series GSE16495 Query DataSets for GSE16495
Status Public on May 28, 2010
Title Expression data from poplar apices
Platform organism Populus sp.
Sample organism Populus tremula x Populus alba
Experiment type Expression profiling by array
Summary We studied differences in gene expression between Populus P35S::EBB1 lines and control, affecting plant growth and differentiation, and dormancy. We used microarrays to detail the global program of gene expression underlying morphological and developmental changes driven by overexpression of the EBB1 gene.
By screening activation tagging population, we identified the EARLY BUD-BREAK1 (EBB1) mutant. We positioned the tag, localized a putative candidate gene and verified transcription activation. The activated gene encodes an AP2/ERF transcription factor similar to a small B1 gene-subfamily in Arabidopsis encoding strong regulators of meristem function and lateral organ outgrowth. We fully recapitulated the phenotype by overexpression of the gene into the same genotype under a strong constitutive promoter (P35S::EBB1). We also found that EBB1 transcript abundance in wild type plants increases during the period prior to bud-break, and in response to the hormone cytokinin. Our data indicates that EBB1 plays an important role in the process of bud-break.
 
Overall design Poplar apex was selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous native expression of affected genes in P35S::EBB1 lines and control, in order to increase the resolution of expression profiles inducing the developmental changes in P35S::EBB1. To do that, we selected apex tissue from greenhouse healthy plants.
 
Contributor(s) Busov VB, Yordanov YS
Citation(s) 24951507
Submission date Jun 08, 2009
Last update date Jan 02, 2015
Contact name Victor Busov
E-mail(s) vbusov@mtu.edu
Organization name Michigan Technological University
Department Forest Resources and Environmental Science
Street address 1400 Townsend Drive
City Houghton
State/province MI
ZIP/Postal code 49931-1295
Country USA
 
Platforms (1)
GPL4359 [Poplar] Affymetrix Poplar Genome Array
Samples (4)
GSM414487 P35S::EBB1_Apex_rep1
GSM414488 P35S::EBB1_Apex_rep2
GSM414489 Control_Apex_rep1
Relations
BioProject PRJNA116111

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16495_RAW.tar 19.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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