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Series GSE16488 Query DataSets for GSE16488
Status Public on Jan 25, 2010
Title growth dependent expression of s. aureus mssa476
Organism Staphylococcus aureus subsp. aureus MSSA476
Experiment type Expression profiling by array
Summary Survival and pathogenesis of Staphylococcus aureus in the host requires the ability to respond to changes. Therefore, tight regulation of gene expression via various regulators is essential. Also, the organization of genes in operons is of influence on the regulation of gene expression. Knowledge of gene expression under different conditions and the ability to accurately predict operons are important steps towards understanding the transcriptional regulation, function and pathogenesis.
A whole genome Agilent microarray was developed for the highly virulent, community-acquired MSSA476.During standard growth in a defined medium, we were able to determine four basic gene expression patterns of S. aureus for both virulence and non-virulence genes. In addition, we predicted operon structures by calculating Pearson correlation coefficients of the transcriptional profiles for all adjacent probes over all time points and replicas.
In this study, we have set a basis for the knowledge on gene expression of MSSA476 during growth. Moreover, the correlation of time-dependent transcriptional profiles of adjacent probes seems to be a promising approach to predict operon structures.
 
Overall design Five separate cultures of S. aureus mssa476 were grown. Of each replicate culture, samples were taken at 1, 2, 3, 4, 5, 6 and 9 h post inoculation (p.i.). In total, this amounts to 35 samples (7 time points in 5 replicates).
 
Contributor(s) Smits N, de Leeuw WC, Fluit AC, Pronk TE, Boel E, Breit TM
Citation(s) 20150412
Submission date Jun 08, 2009
Last update date Mar 21, 2012
Contact name Tessa E Pronk
E-mail(s) tessa.pronk@rivm.nl
Organization name RIVM
Department GBO
Lab GBO
Street address A.v Leeuwenhoeklaan 9
City Bilthoven
ZIP/Postal code 3721 MA
Country Netherlands
 
Platforms (1)
GPL8646 UMCU_IBUMAD_mssa476_5741feat_genecops
Samples (35)
GSM414189 mssa_time4_rep_1
GSM414192 mssa_time4_rep_2
GSM414193 mssa_time6_rep_2
Relations
BioProject PRJNA116329

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Supplementary data files not provided
Processed data included within Sample table

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