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Series GSE16454 Query DataSets for GSE16454
Status Public on Dec 17, 2009
Title Gene expression data from small intestines
Organism Mus musculus
Experiment type Expression profiling by array
Summary Rb and E2F are thought to play antagonistic roles in celll proliferation. However, this model is based mostly from in vitro cell culture systems. We used small intestines to test this model in vivo.
We found that deletion of E2f1-3 in the small intestine of mice suppressed the ectopic expression of E2F targets and cell proliferation caused by Rb-deficiency. Surprisingly, E2f1-3 deletion failed to arrest the proliferation of intestinal cells containing an intact Rb gene, and instead led to E2F target derepression and apoptosis.
 
Overall design Total RNA of crypts and villi from wild-type, Rb-/-, E2f1-/-, E2f2-/-, E2f3-/-, E2f1-/-, E2f2-/-, and E2f3-/- small intestines. Small intestines were harvested 7 days after mice were injected intraperitoneally with beta-napthoflavone.
 
Contributor(s) Leone G, Pipas J
Citation(s) 20016602
Submission date Jun 05, 2009
Last update date Feb 11, 2019
Contact name Gustavo Leone
E-mail(s) jean-leon.chong@osumc.edu
Organization name The Ohio State University
Department 840A Biomedical Research Tower
Street address 640 west 12th avenue
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (24)
GSM413579 WT-1-crypts
GSM413580 WT-2-crypts
GSM413581 Rb-1-crypts
Relations
BioProject PRJNA116023

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16454_RAW.tar 86.1 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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