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Series GSE164291 Query DataSets for GSE164291
Status Public on Jan 06, 2021
Title Cell-type specific analysis of physiological action of estrogen in mouse oviducts
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study is to determine the oviduct responds to 17beta-estradiol (E2) in an in vivo environment using mouse models
 
Overall design Adult female mice (C57B6/J, 8-12 weeks old) were purchased from the Jackson (JAX) laboratory. Animals were ovariectomized and left for 14 days to recover and eliminate endogenous steroid hormones . Sesame oil (100 uL) was used as a negative (Vehicle or Veh) control group. 17beta-estradiol (E2) was diluted in 100 uL of 10% ethanol and sesame oil. Ovariectomized females were subcutaneously injected with Veh or E2. Twenty-four hrs after injection, the oviduct was collected for scRNA-seq analysis. Vaginal washes were collected from adult female mice (between 8-12 weeks old) to determine the stage of estrous cycle. At 11:00 h, oviducts from females in estrus were collected for subsequent scRNA-seq analysis.
 
Contributor(s) McGlade E, Herrera G, Stephens K, Olsen S, Winuthayanon S, Monsivais D, Guner J, Demayo F, Lydon J, Winuthayanon W
Citation(s) 33818810
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HD097087 The actions of steroid hormones on oviduct function Washington State University Wipawee "Joy" Winuthayanon
Submission date Jan 05, 2021
Last update date Apr 07, 2021
Contact name Wipawee Winuthayanon
Organization name Washington State University
Department School of Molecular Biosciences
Street address 1770 Stadium Wy
City Pullman
State/province Washington
ZIP/Postal code 99164
Country USA
 
Platforms (2)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM5005815 V24Am
GSM5005816 V24Is
GSM5005817 E24Am
Relations
BioProject PRJNA689805
SRA SRP300494

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Supplementary file Size Download File type/resource
GSE164291_RAW.tar 184.8 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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