|
| Status |
Public on Jan 06, 2021 |
| Title |
Cell-type specific analysis of physiological action of estrogen in mouse oviducts |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study is to determine the oviduct responds to 17beta-estradiol (E2) in an in vivo environment using mouse models
|
| |
|
| Overall design |
Adult female mice (C57B6/J, 8-12 weeks old) were purchased from the Jackson (JAX) laboratory. Animals were ovariectomized and left for 14 days to recover and eliminate endogenous steroid hormones . Sesame oil (100 uL) was used as a negative (Vehicle or Veh) control group. 17beta-estradiol (E2) was diluted in 100 uL of 10% ethanol and sesame oil. Ovariectomized females were subcutaneously injected with Veh or E2. Twenty-four hrs after injection, the oviduct was collected for scRNA-seq analysis. Vaginal washes were collected from adult female mice (between 8-12 weeks old) to determine the stage of estrous cycle. At 11:00 h, oviducts from females in estrus were collected for subsequent scRNA-seq analysis.
|
| |
|
| Contributor(s) |
McGlade E, Herrera G, Stephens K, Olsen S, Winuthayanon S, Monsivais D, Guner J, Demayo F, Lydon J, Winuthayanon W |
| Citation(s) |
33818810 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HD097087 |
The actions of steroid hormones on oviduct function |
Washington State University |
Wipawee "Joy" Winuthayanon |
|
| |
| Submission date |
Jan 05, 2021 |
| Last update date |
Apr 07, 2021 |
| Contact name |
Wipawee Winuthayanon |
| Organization name |
University of Missouri
|
| Department |
OB/GYN & Women's Health
|
| Street address |
1030 Hitt Street
|
| City |
Columbia |
| State/province |
Missouri |
| ZIP/Postal code |
65211 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA689805 |
| SRA |
SRP300494 |
Less...
More...