 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 27, 2021 |
| Title |
Effector differentiation downstream of lineage commitment in ILC1 is driven by Hobit across tissues. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Innate lymphoid cells (ILCs) mediate early immunity against infection and participate in tissue inflammation and homeostasis. It is unclear how ILCs acquire effector function, and whether these mechanisms differ between organs. Through multiplexed single-cell mRNA-sequencing we identified cKit+CD127hiTCF-1hi early differentiation stages of T-bet+ ILC1. These cells were present across different organs and had the potential to mature towards CD127intTCF-1int and CD127-TCF-1- ILC1. Paralleling a gradual loss of TCF-1, differentiating ILC1 forfeited their expansion potential while increasing expression of effector molecules, reminiscent of T cell differentiation in secondary lymphoid organs. The transcription factor Hobit was induced in TCF-1hi ILC1s and was required for their effector differentiation. These findings reveal sequential mechanisms of ILC1 lineage commitment and ILC1 effector differentiation that are conserved across tissues. Our analyses suggest that ILC1 emerge as TCF-1hi cells in the periphery and acquire a spectrum of organ-specific effector phenotypes through a uniform Hobit-dependent differentiation pathway driven by local cues.
|
| |
|
| Overall design |
Single cell mRNA sequencing of liver ILC1 and NK cells from WT and Hobit-deficient mice.
|
| |
|
| Contributor(s) |
Friedrich C, Taggenbrock R, Doucet-Ladevèze R, Golda G, Moenius R, Arampatzi P, Kragten NA, Kreymborg N, Kastenmueller W, Saliba AE, Grün D, van Gisbergen KP, Gasteiger G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 17, 2020 |
| Last update date |
Jul 29, 2021 |
| Contact name |
Antoine-Emmanuel Saliba |
| E-mail(s) |
emmanuel.saliba@helmholtz-hzi.de
|
| Phone |
+49-931-31-81341
|
| Organization name |
Helmholtz Institute for RNA-based Infection Research
|
| Street address |
Josef-Schneider-Straße 2 / D15
|
| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (1) |
|
| Relations |
| BioProject |
PRJNA686131 |
| SRA |
SRP298373 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE163452_C5_2_README.txt |
167 b |
(ftp)(http) |
TXT |
| GSE163452_RAW.tar |
262.3 Mb |
(http)(custom) |
TAR (of H5, TAR) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
