|
| Status |
Public on Oct 05, 2021 |
| Title |
Targeted DamID in C. elegans reveals a direct role for LIN-22 and NHR-25 in antagonising the epidermal stem cell fate |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In this study we adapt targeted DamID (TaDa) for use in C.elegans and employ it to identify targets within the epidermis for the transcription factors LIN-22 and NHR-25 that are key epidermal development regulators.
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| |
|
| Overall design |
Identification of LIN-22 and NHR-25 genome-wide binding by epidermis-specific expression of TF-Dam fusions in C. elegans.
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| |
|
| Contributor(s) |
Katsanos D, Barkoulas M |
| Citation(s) |
35119932 |
| |
| Submission date |
Dec 15, 2020 |
| Last update date |
Feb 23, 2022 |
| Contact name |
Dimitris Katsanos |
| Organization name |
Imperial College London
|
| Department |
Life Sciences
|
| Lab |
Michalis Barkoulas
|
| Street address |
Sir Alexander Fleming building, Imperial College Road
|
| City |
London |
| ZIP/Postal code |
SW7 2AZ |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL22765 |
Illumina HiSeq 4000 (Caenorhabditis elegans) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA685417 |
| SRA |
SRP298019 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE163243_RAW.tar |
45.3 Mb |
(http)(custom) |
TAR (of GFF) |
| GSE163243_wrt-2-lin-22-dam_L2_F-averaged.gatc.chr-FDR0.05.peaks.gff.gz |
27.3 Kb |
(ftp)(http) |
GFF |
| GSE163243_wrt-2-lin-22-dam_L2_F-averaged.gatc.chr.gff.gz |
2.9 Mb |
(ftp)(http) |
GFF |
| GSE163243_wrt-2-lin-22-dam_L4_F-averaged.gatc.chr-FDR0.05.peaks.gff.gz |
27.2 Kb |
(ftp)(http) |
GFF |
| GSE163243_wrt-2-lin-22-dam_L4_F-averaged.gatc.chr.gff.gz |
3.0 Mb |
(ftp)(http) |
GFF |
| GSE163243_wrt-2-nhr-25-dam_L2_F-averaged.chr.gatc-FDR0.05.peaks.gff.gz |
27.9 Kb |
(ftp)(http) |
GFF |
| GSE163243_wrt-2-nhr-25-dam_L2_F-averaged.chr.gatc.gff.gz |
2.9 Mb |
(ftp)(http) |
GFF |
| GSE163243_wrt-2-nhr-25-dam_L4_averaged.chr.gatc-FDR0.05.peaks.gff.gz |
29.6 Kb |
(ftp)(http) |
GFF |
| GSE163243_wrt-2-nhr-25-dam_L4_averaged.chr.gatc.gff.gz |
3.0 Mb |
(ftp)(http) |
GFF |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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