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| Status |
Public on Dec 31, 2020 |
| Title |
Integrated analysis of lncRNA and mRNA expression profiles in the submandibular glands of DIO mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
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| Summary |
Objective: Obesity contributes to the dysfunction of salivary gland. To explore the specific underlying mechanism for obesity-induced hyposalivation, a model for high-fat diet-induced obese (DIO) mice were constructed to analyze long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles.
Methods: The DIO group and control group were fed a diet containing 60 kcal% fat and a normal chow diet for 16 weeks respectively. Microarray analyses were performed to detect the expression profiles of lncRNA and mRNA in submandibular gland tissues from control group mice and DIO mice. Gene ontology, kyoto encyclopedia of genes and genomes, protein-protein interaction, coding-non-coding gene co-expression, transcription factors and competing endogenous RNA analyses were performed to examine the function of differential expressed genes.
Results: The microarray identified that 624 lncRNAs, along with 297 mRNAs were differentially expressed. Bioinformatic analyses revealed that “complement and coagulation cascades”, “glutathione metabolism”, “cysteine and methionine metabolism”, and “estrogen signaling pathway” were significantly associated with candidate lncRNAs, transcription factors analysis on candidate lncRNAs reveled several genes such as tribbles pseudokinase 3 may play regulatory roles.
Conclusions: Our results revealed the expression profiles of lncRNAs and mRNAs and provided new insights into the mechanism of obesity-induced hyposalivation using bioinformatic analyses.
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| Overall design |
Huimin, Liu
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| Contributor(s) |
Xiang R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 09, 2020 |
| Last update date |
Apr 01, 2021 |
| Contact name |
huimin liu |
| E-mail(s) |
1811210002@pku.edu.cn
|
| Phone |
13146619655
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| Organization name |
Peking University
|
| Department |
Peking University Health Science Center
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| Lab |
Department of Physiology and Pathophysiology
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| Street address |
38 Xueyuan Rd , Haidian District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
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| Platforms (1) |
| GPL26962 |
Agilent-085983 Arraystar Mouse lncRNA V4 microarray |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA683901 |
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