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| Status |
Public on Dec 14, 2020 |
| Title |
Invalidation of a novel marker for therapy-resistant leukemic stem cells responsible for relapse in patients and PDX |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Drug tolerant leukemic cell stem (LSC) subpopulations may explain frequent relapses in acute myeloid leukemia (AML), suggesting that these Relapse-Initiating Cells (RICs) persistent after chemotherapy represent bona fide targets to prevent drug resistance and relapse. We uncover that the G-protein coupled receptor CALCRL is expressed in RICs, and that the overexpression of CALCRL and/or of its ligand adrenomedullin (ADM) and not CGRP correlates to adverse outcome in AML. CALCRL knockdown impairs leukemic growth, decreases LSC frequency and sensitizes to cytarabine in patient-derived xenograft models.
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| |
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| Overall design |
Transfection of MOLM14 cells using genetic approach with shRNA constructs
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| |
|
| Contributor(s) |
Kaoma T, Nicot N, Larrue C, Sarry J |
| Citation(s) |
33462236 |
| Submission date |
Dec 03, 2020 |
| Last update date |
Feb 01, 2021 |
| Contact name |
Tony KAOMA |
| E-mail(s) |
tony.kaoma@lih.lu
|
| Organization name |
Luxembourg Institute of Health
|
| Department |
Quantitative Biology Unit
|
| Lab |
Bioinfo platform
|
| Street address |
1 A-B Rue Thomas Edison
|
| City |
Strassen |
| ZIP/Postal code |
L-1445 |
| Country |
Luxembourg |
| |
|
| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (8)
|
| GSM4955662 |
MOLM14 cell transfected with shcontrol rep1 |
| GSM4955663 |
MOLM14 cell transfected with shcontrol rep2 |
| GSM4955664 |
MOLM14 cell transfected with shcontrol rep3 |
| GSM4955665 |
MOLM14 cell transfected with shcontrol rep4 |
| GSM4955666 |
MOLM14 cell transfected with shCALCRL rep1 |
| GSM4955667 |
MOLM14 cell transfected with shCALCRL rep2 |
| GSM4955668 |
MOLM14 cell transfected with shCALCRL rep3 |
| GSM4955669 |
MOLM14 cell transfected with shCALCRL rep4 |
|
| Relations |
| BioProject |
PRJNA682428 |
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