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Series GSE162562 Query DataSets for GSE162562
Status Public on May 01, 2021
Title Immune transcriptomes of highly exposed SARS-CoV-2 asymptomatic seropositive versus seronegative individuals from the Ischgl community
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary SARS-CoV-2 infection ranges from asymptomatic to severe with lingering symptomatology in some. This prompted investigation of whether or not asymptomatic disease results in measurable immune activation post-infection. Immune activation following asymptomatic SARS-CoV-2 infection was characterized through a comparative investigation of the immune cell transcriptomes from 43 asymptomatic seropositive and 52 highly exposed seronegative individuals from the same community four to six weeks following a superspreading event. Few of the 95 individuals had underlying health issues. One seropositive individual reported Cystic Fibrosis and one individual reported Incontinentia pigmenti. No evidence of immune activation was found in asymptomatic seropositive individuals with the exception of the Cystic Fibrosis patient. There were no statistically significant differences in immune transcriptomes between asymptomatic seropositive and highly exposed seronegative individuals. Four positive controls, mildly symptomatic seropositive individuals whose blood was examined three weeks following infection, showed immune activation. Negative controls were four seronegative individuals from neighboring communities without COVID19. All individuals remained in their usual state of health through a five-month follow-up after sample collection. In summary, whole blood transcriptomes identified individual immune profiles within a community population and showed that asymptomatic infection within a super-spreading event was not associated with enduring immunological activation.
Overall design RNA-seq were performed with peripheral blood mononuclear cells (PBMCs) of 43 asymptomatic COVID-19 patients (A_sample#), 52 highliy exposed seronegative subjects (B_sample#), 5 healthy controls (C_sample#), 4 non-Ischgl COVID-19 patients with mild symtoms (D_sample#) and 4 highly exposed seronegative non-Ischgl subjects (E_sample#).
Contributor(s) Lee H, Knabl L
Citation(s) 33608566, 34100027
Submission date Dec 02, 2020
Last update date Apr 20, 2022
Contact name Hye Kyung Lee
Phone 301-435-6635
Organization name National Institutes of Health (NIH)
Department National Institute of Diabetes and Digestive and Kidney (NIDDK)
Lab Laboratory of Genetics and Physiology
Street address 8 CENTER DR RM 107
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (108)
GSM4954457 A_1_Asymptom
GSM4954458 A_20_Asymptom
GSM4954459 A_57_Asymptom
BioProject PRJNA682211
SRA SRP295561

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Supplementary file Size Download File type/resource
GSE162562_RAW.tar 11.7 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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