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| Status |
Public on Jan 29, 2021 |
| Title |
Contribution of resident and circulating precursors to tumor-infiltrating CD8+ T cell populations in lung cancer [scRNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The goal of this study is to delineate the processes of CD8+ T cell differentiation in primary NSCLC by integrating transcriptomic and tcr profiles.
Methods: A combination of single cell RNA and TCR sequencing (scRNA-seq and scTCR- seq) was used, in tumors, normal tissues adjacent to the tumor (juxta-tumor), and circulating blood derived from 11 patients with untreated, primary NSCLC.
Results: We show that precursor, memory-like CD8+ TILs include 2 main populations: one is also present in the blood (circulating precursors); the other is also present in juxta-tumor tissue and bears markers of memory resident T cells (resident precursors). Both precursor subtypes differentiate into a main population of terminal effectors through a similar “transitional” stage. Terminal effectors are not observed in blood or juxta-tumor tissue, are more clonally expanded, and express signatures of exhaustion. A significant proportion of transitional and terminal effectors also express cell cycle signatures and Ki67, suggesting that clonal expansion occurs in situ is part of the terminal differentiation process.
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| Overall design |
11 patients with early-stage, untreated NSCLC
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| |
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| Contributor(s) |
Gueguen P, Metoikidou C |
| Citation(s) |
33514641 |
| |
| Submission date |
Dec 02, 2020 |
| Last update date |
Feb 23, 2023 |
| Contact name |
Paul Gueguen |
| E-mail(s) |
paul.gueguen@unil.ch
|
| Organization name |
Institut Curie
|
| Department |
Inserm U932
|
| Street address |
26 rue D'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (17)
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| This SubSeries is part of SuperSeries: |
| GSE162500 |
Contribution of resident and circulating precursors to tumor-infiltrating CD8+ T cell populations in lung cancer |
|
| Relations |
| BioProject |
PRJNA682052 |
| SRA |
SRP295502 |
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