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| Status |
Public on Jan 01, 2023 |
| Title |
ASPSCR1-TFE3 reprograms transcription by organizing enhancers around hexameric VCP [hASPS_ChIP, ASPL_VCP_Pol2] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Aberrant transcription in alveolar soft part sarcoma (ASPS) is orchestrated by the fusion oncoprotein, ASPSCR1-TFE3, product of a gene fusion created by a t(X;17) chromosomal translocation. Here, proteomics of proteins co-immunoprecipitating with ASPSCR1-TFE3 revealed strong enrichment of VCP/p97, an AAA+ ATPase with known segregase function. Positive and negative genetic experiments with ASPSCR1-TFE3 and VCP demonstrated that they function co-dependently for cancer cell proliferation, colony formation, enhancer activation, chromatin conformation, and transcription
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| Overall design |
ASPS cell lines, tumor from ASPS mouse model and tumor from ASPS patients are used for ASPSCR1, VCP, POLII and various histone modification
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| |
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| Citation(s) |
38326311 |
| |
| Submission date |
Dec 01, 2020 |
| Last update date |
Feb 15, 2024 |
| Contact name |
Jinxiu Li |
| Organization name |
China Agricutural University
|
| Department |
College of biological sciences
|
| Lab |
State key laboratories of Agrobiotechnology
|
| Street address |
Yuanmingyuan west road 2#
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE162609 |
ASPSCR1-TFE3 reprograms transcription by organizing enhancers around hexameric VCP |
|
| Relations |
| BioProject |
PRJNA681932 |
| SRA |
SRP295444 |
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