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| Status |
Public on Feb 25, 2021 |
| Title |
Time-resolved single cell transcriptomics of an ibrutinib-treated mantle cell lymphoma cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed a high-throughput droplet-based single-cell RNA sequencing experiment on a sensitive MCL cell line model (REC-1) treated with ibrutinib over time using the 10x Genomics platform. Two biological replicates were generated of an untreated control, 6 h, and 48 h ibrutinib-treated cells.
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| Overall design |
REC-1 cells were cultured for 48 h, one sample stayed untreated, the second was treated with 400 nM ibrutinib for 6 h and the third for 48 h. After harvesting, the cells were stained with propidium iodide to identify the surviving cells which entered single-cell RNA sequencing.
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| Contributor(s) |
Fuhr V, Vafadarnejad E, Dietrich O, Arampatzi P, Riedel A, Saliba AE, Rosenwald A, Rauert-Wunderlich H |
| Citation(s) |
33668876 |
| |
| Submission date |
Nov 27, 2020 |
| Last update date |
Mar 08, 2021 |
| Contact name |
Antoine-Emmanuel Saliba |
| E-mail(s) |
emmanuel.saliba@helmholtz-hzi.de
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| Phone |
+49-931-31-81341
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| Organization name |
Helmholtz Institute for RNA-based Infection Research
|
| Street address |
Josef-Schneider-Straße 2 / D15
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| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA681209 |
| SRA |
SRP294496 |
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