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Series GSE16162 Query DataSets for GSE16162
Status Public on May 20, 2009
Title Differential expression of whole transcriptome in Synechocystis sp. PCC 6803
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary The model cyanobacterium Synechocystis sp. PCC 6803 was used for a systematic survey of differential expression with a focus on antisense (as)RNAs and non-coding (nc)RNAs. A microarray was constucted with on average 5 probes for each transcript known thus far, including ncRNAs and asRNAs. The resulting 20,431 individual probes are duplicated on the array (Agilent 4x44k custom array) representing a technical replicate.
Hybridization of this array with total RNA isolated from cultures raised under different growth conditions identified transcripts from intergenic spacers and in antisense orientation to known genes (natural cis-asRNAs) with differential expression compared to control hybridizations. This shows the involvement of such transcripts in the regulation of adaptation to various stresses.
 
Overall design 12 RNA hybridizations (1 control & 3 stress conditions, 3 times each)
 
Contributor(s) Georg J, Voss B, Scholz I, Mitschke J, Wilde A, Hess W
Citation(s) 21245330, 19756044
Submission date May 19, 2009
Last update date Sep 12, 2014
Contact name Bjoern Voss
Organization name University of Freiburg
Department Institute of Biology III
Lab Genetics
Street address Schänzlestr. 1
City Freiburg
ZIP/Postal code 79104
Country Germany
 
Platforms (1)
GPL8567 Agilent-021974 Syn_PCC6803_transcriptome
Samples (12)
GSM405544 Control_1
GSM405545 Control_2
GSM405546 Control_3
Relations
BioProject PRJNA117187

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16162_RAW.tar 57.5 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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