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| Status |
Public on Jan 04, 2021 |
| Title |
Deletion of the helicase RTEL1 in RNA:DNA hybrids throughout the genome and how this may be affected by overexpression of the protein WT RNaseH1GFP in MEFs |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
The goal of this study is to assess the effect of the deletion of the helicase RTEL1 in RNA:DNA hybrids throughout the genome and how this may be affected by overexpression of the protein WT RNaseH1GFP in mouse embryonic fibroblasts.
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| Overall design |
We used RTEL1F/F mouse embryonic fibroblasts that upon infection with Cre adenoparticles delete Rtel1 and upon addition of doxycycline overexpress WT RNaseH1GFP (Rtel1F/F;WT RNH1-GFP MEFs). Rtel1F/F;WT RNH1-GFP MEFs were infected with GFP or Cre-GFP adenovirus, after 48 h doxycycline (2 μg/ml) was added and cells were collected after 48 h. RNA:DNA hybrids were pulled down using the DRIP protocol and DNA was isolated and send for sequencing.
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| Contributor(s) |
Margalef P, Kotsantis P, Segura-Bayona S, Poetsch AR, Patel H, Ruis P, Marzec P, Bellelli R, Hewitt G, Stanage TH, Goldstone R, Boulton SJ |
| Citation(s) |
33357438 |
| |
| Submission date |
Nov 17, 2020 |
| Last update date |
Jan 04, 2021 |
| Contact name |
Simon J Boulton |
| E-mail(s) |
simon.boulton@crick.ac.uk
|
| Organization name |
The Francis Crick Institute
|
| Lab |
DSB Repair Metabolism Lab
|
| Street address |
1 Midland Rd
|
| City |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (8)
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| GSM4910678 |
Rtel1f/f Ad-Cre +DOX DRIP biological replicate 1 |
| GSM4910679 |
Rtel1f/f Ad-Cre MOCK DRIP biological replicate 1 |
| GSM4910680 |
Rtel1f/f Ad-GFP +DOX DRIP biological replicate 1 |
| GSM4910681 |
Rtel1f/f Ad-GFP MOCK DRIP biological replicate 1 |
| GSM4910682 |
Rtel1f/f Ad-Cre +DOX Input biological replicate 1 |
| GSM4910683 |
Rtel1f/f Ad-Cre MOCK Input biological replicate 1 |
| GSM4910684 |
Rtel1f/f Ad-GFP +DOX Input biological replicate 1 |
| GSM4910685 |
Rtel1f/f Ad-GFP MOCK Input biological replicate 1 |
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| This SubSeries is part of SuperSeries: |
| GSE161597 |
RTEL1 regulates G4/R-loops to avert replication-transcription collisions |
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| Relations |
| BioProject |
PRJNA678869 |
| SRA |
SRP292869 |
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