|
| Status |
Public on Nov 16, 2020 |
| Title |
Genomic occupancy of MYC and members of the PRC1.6 complex (MGA, E2F6, MAX, L3MBTL2) in MGA WT (Empty) and MGA depleted (sgMga) KrasG12D Trp53 -/- mouse lung cancer lines |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Conventional crosslinked ChIP was performed on cell lines derived from control (Empty) and sgMga transduced tumors. We wanted to assess PRC1.6 member and MYC binding in the presence and absence of MGA.
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| |
|
| Overall design |
Conventional X-ChIP was performed on Empty and sgMga KP mouse lung cancer lines. Antibodies used: IgG control rabbit, IgG control mouse, anti-MAX, anti-MYC, anti-MGA, anti-L3MBTL2, anti-E2F6)
|
| |
|
| Contributor(s) |
Mathsyaraja H, Eisenman R |
| Citation(s) |
34236315 |
| |
| Submission date |
Nov 16, 2020 |
| Last update date |
Jul 20, 2021 |
| Contact name |
Haritha Mathsyaraja |
| E-mail(s) |
hmathsya@fredhutch.org
|
| Organization name |
Fred Hutch
|
| Department |
Basic Sciences
|
| Lab |
Robert N Eisenman
|
| Street address |
1100 Fairview Ave N
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (14)
|
|
| Relations |
| BioProject |
PRJNA678678 |
| SRA |
SRP292710 |
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