|
| Status |
Public on Nov 13, 2020 |
| Title |
Identification of genes responsive to hypoxia in human cervical epithelial adenocarcinoma HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
It has been well-known that cancer cells acquire approximately three-fold radioresistance under hypoxia than under normoxia due to both radiation chemical and radiation biological mechanisms, causing a negative impact on the outcome of radiation therapy.
In the present study, to identify genes responsive to hypoxia in human cervical epithelial adenocarcinoma HeLa cells, global-scale gene expression analysis was carried out using a GeneChip® system.
|
| |
|
| Overall design |
Human cervical carcinoma cell line HeLa cells were used. Cells were cultured at 37°C in Dulbecco’s modified Eagle medium (DMEM) containing 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. Cells were incubated in a well-humidified 5% CO2 and 95% air for the normoxic culture, or in a RUSKINN INVIVO2 500 for hypoxic culture at < 0.1% O2. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with a Human Genome U133-plus 2.0 array. Sample preparation for array hybridization was carried out as described in the manufacturer’s instructions.
|
| |
|
| Contributor(s) |
Tabuchi Y, Harada H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 12, 2020 |
| Last update date |
Nov 15, 2020 |
| Contact name |
Yoshiaki Tabuchi |
| E-mail(s) |
ytabu@cts.u-toyama.ac.jp
|
| Organization name |
University of Toyama
|
| Department |
Life Science Research Center
|
| Lab |
Division of Molecular Genetics Research
|
| Street address |
2630 Sugitani
|
| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA678012 |
