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| Status |
Public on Sep 01, 2021 |
| Title |
Genome wide analysis of Suv4-20h1/2 depleted animal caps [dataset3] |
| Organism |
Xenopus tropicalis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Suv4-20h1/2 are histone methyltransferases that write the H4K20me2 and H4K20me3 marks. We knocked down these enzymes using translation blocking morpholinos in X. tropicalis animal caps and performed RNA-seq in order to investigate the impact of altering H4K20me state on epidermal differentiation. Knocking down Suv4-20h1/2 leads to a strong increase of H4K20me1 in bulk chromatin. To determine whether increased H4K20me1 is responsible for transcriptional changes in suv4-20h KD animal caps, we performed RNA-Seq for a rescue experiment with PHF8, an H4K20me1 demethylase.
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| Overall design |
RNA-seq experiment of human Phf8 mRNA injection in Suv4-20H1/2 knock down or control conditions (i.e. control morpholino, LacZ mRNA) in 3 biological replicates.
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| Contributor(s) |
Angerilli A, Tait J, Schauer T, Shcherbakova I, Rupp R |
| Citation(s) |
37116939 |
| |
| Submission date |
Nov 11, 2020 |
| Last update date |
Aug 02, 2023 |
| Contact name |
Tamas Schauer |
| E-mail(s) |
tamas.schauer@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum München
|
| Department |
Institute of Epigenetics and Stem Cells
|
| Street address |
Feodor-Lynen-Straße 21
|
| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL29398 |
Illumina HiSeq 1500 (Xenopus tropicalis) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE161251 |
Genome wide analysis of Suv4-20h1/2 depleted animal caps |
|
| Relations |
| BioProject |
PRJNA677828 |
| SRA |
SRP292402 |
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