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| Status |
Public on Nov 10, 2023 |
| Title |
Serca2b inhibition aids T cell activation and selectively promotes tumor cell death |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We found that T cells and tumor cells are differentially dependent on Atp2a2 for their survival. In fact, Atp2a2 KO T cells have increased function, whereas Atp2a2 KO tumor cells die. Thapsigargin is a drug that targets the protein product of Atp2a2: Serca2b. We found that in tumor cells, the SAGA complex transmits TG signals to induce tumor cell death, principally via Ccdc101.
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| Overall design |
Here, we treated tumor cells (either WT or Ccdc101 KO) or T cells (WT or Atp2a2 KO) with TG (50ng/mL) or Veh (DMSO) for 8 hours. The T cells were additionally activated with anti-CD3 antibodies (2.5ug/mL in PBS) for 24 hours (Act) or not (Rest).
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| Contributor(s) |
Vredevoogd D, Krijgsman O, Peeper D |
| Citation missing |
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| |
| Submission date |
Nov 10, 2020 |
| Last update date |
Nov 10, 2023 |
| Contact name |
Oscar Krijgsman |
| E-mail(s) |
o.krijgsman@nki.nl, oscarkrijgsman@gmail.com
|
| Phone |
31205122028
|
| Organization name |
Netherlands Cancer Institute
|
| Department |
Molecular Oncology and Immunology
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| Lab |
Peeper
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| Street address |
Plesmanlaan 121
|
| City |
Amsterdam |
| State/province |
Outside the US or Canada |
| ZIP/Postal code |
1066CX |
| Country |
Netherlands |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA675875 |
| SRA |
SRP292036 |
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