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| Status |
Public on Nov 01, 2021 |
| Title |
The influence of nt-LNA on cell composition and phenotypees in the atherosclerotic lesion. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Raw fastq files from paired-end, Illumina-based sequencing on NovaSeq6000
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| Overall design |
Apoe-/- mice were fed a western diet for 4 weeks and administered saline or nt-LNA (30 mg/kg) weekly. After 4 weeks, aortic arch and thoracic aorta were removed, cleaned of peripheral tissue, minced and digested to achieve a single cell suspension. The single cell suspension was then sorted by FACS to achieve a CD45+/CD3- population of live cells for single-cell 3' RNA Sequencing utilizing the 10X Chromium system.
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| |
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| Contributor(s) |
Allen RM, Vickers KC, Michell DL |
| Citation(s) |
36474072 |
| |
| Submission date |
Nov 04, 2020 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Ryan M Allen |
| E-mail(s) |
ryan.m.allen@vumc.org
|
| Phone |
3146109328
|
| Organization name |
Vanderbilt University Medical Center
|
| Department |
Medicine
|
| Lab |
Vickers
|
| Street address |
2220 Pierce Ave
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (2) |
| GSM4879344 |
Single-cell transcriptomes of macrophages isolated from atherosclerotic lesions of saline-treated mice |
| GSM4879345 |
Single-cell transcriptomes of macrophages isolated from atherosclerotic lesions of nt-LNA-treated mice |
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| This SubSeries is part of SuperSeries: |
| GSE206859 |
LDL delivery of microbial small RNAs drives atherosclerosis through macrophage TLR8 |
|
| Relations |
| BioProject |
PRJNA674509 |
| SRA |
SRP291094 |
