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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 22, 2009 |
| Title |
Expression Data from Pten Null Fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The tumor stroma is believed to contribute to some of the most malignant characteristics of epithelial tumors. However, signaling between stromal and tumor cells is complex and remains poorly understood. Here we show that genetic inactivation of Pten in stromal fibroblasts of mouse mammary glands accelerated the initiation, progression and malignant transformation of mammary epithelial tumors.
Global gene expression profiling in mammary stromal cells identified a Pten-specific signature associated with massive extra-cellular matrix (ECM) remodeling, innate immune cell infiltraion and increased angiogenesis. Execution of this transcriptional program was mediated, in part, by the induction, phosphorylation and recruitment of Ets2 to target promoters. Remarkably, Ets2 inactivation in Pten stroma-deleted tumors was sufficient to decrease tumor growth and progression. These findings identify the Pten-Ets2 axis as a critical stroma-specific signaling pathway that suppresses mammary epithelial tumors.
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| Overall design |
Wild type and Pten null primary mammary fibroblasts isolated from 8 week old female mice were cultured, RNA was extracted and Affymetrix gene expression arrays were performed.
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| Contributor(s) |
Stone CZ, Wallace JA, Kornacker K, Leone G, Ostrowski MC |
| Citation(s) |
19847259 |
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| Submission date |
May 12, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Julie Wallace |
| E-mail(s) |
Julie.Reeser@osumc.edu
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| Organization name |
OSUCCC
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| Street address |
460 W. 12th Ave 840 BRT
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| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM402452 |
Pten null fibroblasts, biological rep1 |
| GSM402453 |
Pten null fibroblasts, biological rep2 |
| GSM402454 |
Pten null fibroblasts, biological rep3 |
| GSM402455 |
Wild type fibroblasts, biological rep1 |
| GSM402456 |
Wild type fibroblasts, biological rep2 |
| GSM402457 |
Wild type fibroblasts, biological rep3 |
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| Relations |
| BioProject |
PRJNA115499 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16073_RAW.tar |
21.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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