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| Status |
Public on Jul 07, 2022 |
| Title |
Single-cell RNA-sequencing of immune cells (CD45+) from the tumor microenvironment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In mouse models of PDGF-driven glioma where Notch is tumor-suppressive, we find that Notch activity shapes interferon-response in tumor cells and the local immune microenvironment early during tumor progression. Notch inhibition disturbs cytokine expression by tumor cells, altering recruitment and activation of immune cell populations and favoring hyper-proliferative "immune niche" independent growth. Genetic inactivation of Notch in glioma cells also attenuates their response to interferon-gamma establishing a non-cell autonomous inhibitory feedback loop that further promotes immune evasion and aggressiveness. Hence, we propose that Notch signaling levels in tumor cells are key to orchestrate immune escape and microenvironment independency during brain tumor formation.
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| Overall design |
CD45+ immune cells from pools of 9 healthy brains, 15 control tumors and 9 Rbpj-/- tumors were purified by FACS and analyzed by single cell RNA-seq.
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| Contributor(s) |
Ivanek R, Parmigiani E, Taylor V, Giachino C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 03, 2020 |
| Last update date |
Jul 07, 2022 |
| Contact name |
DBM Bioinformatics Core Facility |
| Phone |
+41612073541
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| Organization name |
University of Basel
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| Department |
Departement of Biomedicine
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| Street address |
Hebelstrasse 20
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| City |
Basel |
| State/province |
BS |
| ZIP/Postal code |
4053 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE160715 |
Notch activity in tumor cells shapes interferon-gamma response and immune microenvironment in glioma |
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| Relations |
| BioProject |
PRJNA674066 |
| SRA |
SRP290954 |
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