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Series GSE160585 Query DataSets for GSE160585
Status Public on Jan 18, 2021
Title Vascular smooth muscle-derived TRPV1-expressing progenitors are a new source of cold induced thermogenic adipocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Brown adipose tissue (BAT) functions in energy expenditure in part due its role in thermoregulation. The prominent capacity of BAT to enhance fuel utilization and energy expenditure makes it an attractive target for treating obesity and metabolic disorders. Prolonged cold exposure induces de novo recruitment of brown adipocytes and activates their thermogenic activity. However, the exact source of cold-induced brown adipocytes is not completely understood. In this study, we sought to investigate the cellular origin of cold-induced brown adipocytes using single-cell RNA sequencing. We identified two distinct types of adipocyte progenitors that contribute to de novo recruitment of brown adipocytes in response to cold challenge. One is the previously known Pdgfra-expressing mesenchymal progenitors and the other is a vascular smooth muscle-derived adipocyte progenitor (VSM-APC) population, which expresses the temperature-sensitive ion channel transient receptor potential cation channel subfamily V member 1 (Trpv1). Using flow cytometry and lineage tracing, we demonstrated that the Trpv1pos VSM-APCs were indeed distinct from the Pdgfrapos progenitors and could contribute to brown adipocytes with greater thermogenic potential. Together, these findings illustrate a landscape of thermogenic adipose niche at the single cell resolution and identify a new cellular origin for the development of brown adipocytes.
Overall design To characterize the cellular composition of the BAT niche during cold-induced remodeling, we applied scRNA-seq to cells isolated from the stromal vascular fraction of BAT (BAT-SVF) from mice housed at either thermoneutral (TN: 30 °C for 1 week), room temperature (RT: 22 °C) or cold (5 °C for 2 days or 7 days).
Contributor(s) Tseng Y, Shamsi F, Piper M
Citation(s) 33846638
NIH grant(s)
Grant ID Grant title Affiliation Name
K01 DK125608 The role of vascular endothelium in BAT expansion and remodeling JOSLIN DIABETES CENTER Farnaz Shamsi
R01 DK077097 Regulation of human brown and beige adipocyte differentiation and function JOSLIN DIABETES CENTER Yu-Hua Tseng
R01 DK102898 Fibroblast Growth Factor and Energy Metabolism JOSLIN DIABETES CENTER Yu-Hua Tseng
R01 DK122808 Role of brown fat-derived specialized pro-resolving lipid mediators in inflammation and metabolism JOSLIN DIABETES CENTER Yu-Hua Tseng
K01 DK111714 The Lipidomics of Adipose Tissue Thermogenesis JOSLIN DIABETES CENTER MATTHEW LYNES
Submission date Nov 01, 2020
Last update date Apr 19, 2021
Contact name Yu-Hua Tseng
Organization name Joslin Diabetes Center Harvard Medical School
Street address 1 Joslin Place
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (16)
GSM4875674 TN_1
GSM4875675 TN_2
GSM4875676 RT_1
BioProject PRJNA673699
SRA SRP290688

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE160585_filtered_raw_counts_all_clusters.tar.gz 523.2 Mb (ftp)(http) TAR
GSE160585_filtered_raw_counts_for_pseudotime_and_pseudobulk_DGE.tar.gz 180.1 Mb (ftp)(http) TAR
GSE160585_metadata_all_clusters.csv.gz 7.2 Mb (ftp)(http) CSV
GSE160585_metadata_for_pseudotime_and_pseudobulk_DGE.csv.gz 1.7 Mb (ftp)(http) CSV
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Processed data are available on Series record

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