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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 29, 2020 |
| Title |
Cells in healthy periodontal ligament |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Loss of tissue attachment as a consequence of bacterial infection and inflammation represents the main therapeutic target for the treatment of periodontitis. Cementoblasts, the cells that produce the mineralized tissue, cementum, that is responsible for connecting the soft periodontal tissue to the tooth, are a key cell type for maintaining/restoring tissue attachment following disease. Here, we identify two distinct stem cell populations that contribute to cementoblast differentiation at different times by lineage tracing and single cell RNA sequencing. During postnatal development, cementoblasts are formed from perivascular‐derived cells expressing CD90 and perivascular‐associated cells that express Axin2. During adult homeostasis, only Wnt‐responsive Axin2+ cells form cementoblasts but following experimental induction of periodontal disease, CD90+ cells become the main source of cementoblasts. We thus show that different populations of resident stem cells are mobilized at different times and during disease to generate precursors for cementoblast differentiation and thus provide an insight into the targeting cells resident cells for novel therapeutic approaches.
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| Overall design |
Mouse PDL mRNA profiles during adult homeostasis.
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| Contributor(s) |
Zhao J, Faure L, Adameyko I, Sharpe P |
| Citation(s) |
33038290, 36193890 |
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| Submission date |
Oct 28, 2020 |
| Last update date |
Oct 28, 2022 |
| Contact name |
Paul Sharpe |
| Organization name |
King's college London
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| Street address |
Craniofacial Development & Stem cell bio, Guy's Tower Floor 27
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| City |
london |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (2) |
| GSM4872144 |
Periodontal ligament tissue homeostasis 1 |
| GSM4872145 |
Periodontal ligament tissue homeostasis 2 |
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| Relations |
| BioProject |
PRJNA672992 |
| SRA |
SRP289556 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE160358_RAW.tar |
5.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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