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| Status |
Public on Oct 29, 2020 |
| Title |
Macrophage expression and prognostic significance of the long pentraxin PTX3 in COVID-19 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
PTX3 is an essential component of humoral innate immunity, involved in resistance to selected pathogens and in the regulation of inflammation1-3. The present study was designed to assess the presence and significance of PTX3 in COVID-194-7. RNA-seq analysis of peripheral blood mononuclear cells, single-cell bioinformatics analysis and immunohistochemistry of lung autopsy samples revealed that myelomonocytic cells and endothelial cells express high levels of PTX3 in COVID-19 patients. Increased plasma concentrations of PTX3 were detected in 96 COVID-19 patients. PTX3 emerged as a strong independent predictor of 28-day mortality in multivariable analysis, better than conventional markers of inflammation, in hospitalized COVID-19 patients. The prognostic significance of PTX3 abundance for mortality was confirmed in a second independent cohort (54 patients). Thus, circulating and lung myelomonocytic cells and endothelial cells are a major source of PTX3, and PTX3 plasma concentration can serve as an independent strong prognostic indicator of short-term mortality in COVID-19.
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| Overall design |
Monocytes were isolated from peripheral blood samples of three healthy donors and six COVID-19 patients and RNA expression was measured with total RNA-Seq.
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| Contributor(s) |
Mantovani A, Garlanda C, Bottazzi B, Mapelli SN |
| Citation(s) |
33208929, 34145258 |
| |
| Submission date |
Oct 28, 2020 |
| Last update date |
Jul 08, 2021 |
| Contact name |
sarah natalia mapelli |
| E-mail(s) |
sarah.mapelli@humanitasresearch.it
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| Organization name |
Humanitas research
|
| Department |
Experimental Immunopathology
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| Lab |
Experimental Immunopathology
|
| Street address |
Via Rita Levi Montalcini 4
|
| City |
Pieve Emanuele |
| State/province |
Milan |
| ZIP/Postal code |
20090 |
| Country |
Italy |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA672981 |
| SRA |
SRP289541 |
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