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| Status |
Public on Jun 18, 2021 |
| Title |
RNA polymerase II is necessary for spatial chromatin reorganization following exit from mitosis [ATAC-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Mammalian chromosomes are three-dimensional entities shaped by converging and opposing forces acting on chromatin. Mitotic cell division induces drastic chromatin condensation, but following reentry into the G1 cell cycle phase, condensed chromosomes unwind to re-establish interphase organization. Here, we use a cell line allowing auxin-mediated degradation of RNA polymerase II to test its role in this transition. In situ Hi-C and super-resolution imaging showed that RNAPII is required for compartment and loop formation following mitosis. RNAPs also often counteract loop extrusion and, in their absence, longer and more prominent loops arise. Evidence from chromatin fractionation and in silico modeling attribute these effects to RNAPII-mediated cohesin loading at active promoters upon G1 reentry. Our findings reconcile the role of RNAPII in gene expression with that in chromatin architecture.
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| |
|
| Overall design |
Assay for Transposase-Accessible Chromatin using sequencing in DLD1 cells
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| |
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| Contributor(s) |
Papantonis A, Übelmesser N, Josipovic N |
| Citation(s) |
34166567, 34678064 |
| |
| Submission date |
Oct 28, 2020 |
| Last update date |
Nov 10, 2021 |
| Contact name |
Argyris Papantonis |
| E-mail(s) |
argyris.papantonis@med.uni-goettingen.de
|
| Organization name |
University Medical Center Goettingen
|
| Department |
Department of Pathology
|
| Street address |
Robert-Koch-Str 40
|
| City |
Goettingen |
| ZIP/Postal code |
37075 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE160321 |
RNA polymerase II is necessary for spatial chromatin reorganization following exit from mitosis |
|
| Relations |
| BioProject |
PRJNA672941 |
| SRA |
SRP289481 |
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