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Series GSE160098 Query DataSets for GSE160098
Status Public on Oct 27, 2020
Title Directionality of developing skeletal muscles is set by mechanical forces
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Formation of oriented myofibrils is a key event in the development of a functional musculoskeletal system. However, the mechanisms that control orientation of myocytes, their fusion and the resulting directionality of adult muscles remain enigmatic. Here, we utilized in vivo and in vitro live imaging, CAS9/CRISPR-mediated mutagenesis in fish, genetic experiments in mice and single cell transcriptomics to demonstrate that individual myocyte polarization and subsequent orientation depend on cell stretch imposed by skeletal expansion. Our data revealed that upon migration, individual facial myocytes form unpolarized clusters corresponding to future muscle groups. These clusters undergo oriented stretch and alignment during embryonic growth. Experimental in vivo perturbations of cartilage shape, size and distribution caused disruptions in directionality and number of myofibrils. Controlled in vitro 2D and 3D experiments applying continuous tension via artificial attachment points demonstrated a sufficiency for mechanical forces to instruct coherent polarization of myocyte populations. Consistently, perturbations of cartilage extension revealed a role of the developing skeleton in the directional outgrowth of non-muscle soft tissues during limb and facial morphogenesis.
 
Overall design We performed single cell transcriptomic analysis of cells collected daily from soft and hard culture conditions (Fig. 7b). cDNA libraries were prepared and sequenced from 2000 cells from each condition via 10X Genomics Chromium platform
 
Contributor(s) Sunadome K, Kah D, Fabry B, Adori C, Erickson A, Kameneva P, Faure L, Kanatani S, Kaucka M, Dehnisch I, Tesarova M, Zikmund T, Kaiser J, Edwards S, Fried K, Adameyko  I
Citation(s) 37244931
Submission date Oct 26, 2020
Last update date Aug 30, 2023
Contact name Louis Faure
E-mail(s) louis.faure@meduniwien.ac.at
Phone 06766018048
Organization name Medical University of Vienna
Street address Spitalgasse 4 Uni-Campus, 1090 Wien
City Wien
State/province Österreich
ZIP/Postal code 1090
Country Austria
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (2)
GSM4859875 C2C12 on 0.5Kpa-soft
GSM4859876 C2C12 on 100Kpa-hard
Relations
BioProject PRJNA671829
SRA SRP288573

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE160098_RAW.tar 582.5 Mb (http)(custom) TAR (of H5, LOOM)
GSE160098_p2w_batch_corrected.RData.gz 536.6 Mb (ftp)(http) RDATA
GSE160098_p2w_batch_corrected.bin.dat.gz 209.6 Mb (ftp)(http) DAT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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