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| Status |
Public on Jan 28, 2022 |
| Title |
MAT2A expression and methionine dependency in Diffuse Midline Gliomas creates a distinct metabolic and epi-genetic sensitivity |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We generated a syngeneic H3K27M diffuse midline glioma (DMG) mouse model and performed total RNA seq on H3K27MPP cells and control cell lines H3wtMPP and Normal Neural stem cells. Aditionally we generate a Dox Inducible Mat2A Knockdown in that human DIPG cell line DIPG04 and performed RNA sequencing on cells treated with 2ug/ml Doxycyclin and no treatment
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| Overall design |
Samples werer analyzed in triplicate
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| |
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| Contributor(s) |
Agnihotri S, golbourn B, Habert M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Oct 26, 2020 |
| Last update date |
Jan 28, 2022 |
| Contact name |
Sameer Agnihotri |
| E-mail(s) |
labagnihotri@gmail.com
|
| Phone |
4122188456
|
| Organization name |
University of Pittsburgh
|
| Department |
Neurosurgery
|
| Lab |
Agnihotri
|
| Street address |
530 45TH ST, 7TH FL BAY 20 RANGOS RESEARCH
|
| City |
Pittsburgh |
| State/province |
PENNSYLVANIA |
| ZIP/Postal code |
15201-1136 |
| Country |
USA |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA671811 |
| SRA |
SRP288557 |
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