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| Status |
Public on Jul 22, 2009 |
| Title |
Gene expression signature of naïve and in vitro activated CD8 T cells in response to IL-12 and Type I IFN |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Differentiation of naive CD8 T cells into cytotoxic effector cells requires three distinct signals- antigen (signal 1), costimulation -B7-1 (signal 2) and cytokine, either interleukin-12 or interferon-a/b (signal 3). Interaction of naive CD8 T cells with antigen and B7-1 programs cell division and proliferation whereas the presence of cytokines- IL-12 or IFNa/b promote survival, differentiation and memory establishment. In the absence of signal 3, the cells interacting with antigen/B7-1 undergo tolerance induction. The objective of this study was to elucidate the mechanisms how the provision of signal 3 promotes differentiation and averts tolerance induction in CD8 T cells. Trichostatin A is a pharmacological agent that inhibits histone deacetylase activity, hence regulating chromatin structure and gene expression and differentiation in many cell types. Gene signature profiles of IL-12, IFNa/b and trichostatin A stimulated cells were compared to elucidate the molecular mechanisms of gene regulation.
Oligonucleotide microarray analysis is carried out to determine the extent and molecular nature of the CD8 T cell differentiation program induced by IL-12 or IFNa/b in concert with antigen and B7-1 signal.
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| Overall design |
The programming for development of function and memory in presence of signal 3 occurs over three days of initial stimulation, and antigen-B7 and IL-12 or IFNa/b must be present for most of this period to achieve maximal responses. We analyzed gene expression in highly purified naive CD8 T cells at 0, 24, 48 and 72h of in vitro culture stimulated with antigen-B7 and with or without IL-12 or IFNa/b to tease apart gene expression profiles of naive, 2-signal and 3-signal stimulated cells over the course of 3-days. Gene expression of cells stimulated with trichostatin A for 72hr were compared with IL-12 or IFNa/b stimulated cells. 20 arrays.
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| Contributor(s) |
Agarwal P, Mescher MF |
| Citation(s) |
19592655 |
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| Submission date |
May 01, 2009 |
| Last update date |
Feb 18, 2018 |
| Contact name |
PUJYA AGARWAL |
| E-mail(s) |
agar0050@umn.edu
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| Phone |
612-626-2476
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| Organization name |
University of Minnesota
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| Department |
Laboratory,Medicine and Pathology
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| Street address |
6-246 NHH, 312 Church Street, SE
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| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (20)
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| GSM399649 |
Ag-B7@ 24hr, biological rep1 |
| GSM399650 |
Ag-B7+IL12 @24hr, biological rep1 |
| GSM399651 |
Ag-B7+IFNa/b @24hr, biological rep1 |
| GSM399652 |
Ag-B7@ 48hr, biological rep1 |
| GSM399653 |
Ag-B7@ 48hr, biological rep2 |
| GSM399654 |
Ag-B7@ 48hr, biological rep3 |
| GSM399655 |
Ag-B7@ 48hr, biological rep4 |
| GSM399656 |
Ag-B7+IL12 @48hr, biological rep1 |
| GSM399657 |
Ag-B7+IL12 @48hr, biological rep2 |
| GSM399658 |
Ag-B7+IL12 @48hr, biological rep3 |
| GSM399659 |
Ag-B7+IFNa/b @48hr, biological rep1 |
| GSM399660 |
Ag-B7+IFNa/b @48hr, biological rep2 |
| GSM399661 |
Ag-B7+IFNa/b @48hr, biological rep3 |
| GSM399662 |
Ag-B7@ 72hr, biological rep1 |
| GSM399663 |
Ag-B7+IL12 @72hr, biological rep1 |
| GSM399664 |
Ag-B7+IFNa/b @72hr, biological rep1 |
| GSM399665 |
Ag-B7+TSA @72hr, biological rep1 |
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| Relations |
| BioProject |
PRJNA116943 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15930_RAW.tar |
147.4 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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