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Status |
Public on Jun 28, 2021 |
Title |
RNA Sequencing of PGE2 or MEHP treated human THP-1 derived macrophages transcriptomes |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
THP-1 cells were treated with phorbol 12-myristate 13-acetate (PMA) at 25μg/ml for 48h.Then remove PMA and rest for 24h to obtain M0 macropahges. Further applied LPS and IFNγ or IL-4 and IL-13 for 24h to obtain M1, M2 macrophages, respectively. During polarization, cells were treated with prostaglandin E2 or MEHP.Then M1,M2 cells were collected and applied to RNA-sequencing. We found that PGE2 and MEHP significantly impacted metabolic signature of macrophages.
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Overall design |
mRNA profiles of PGE2 or MEHP treated M1 and M2 macrophages were examined by Illumina Hiseq.
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Contributor(s) |
Yang H, Xu M |
Citation(s) |
34149684, 35019730 |
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Submission date |
Oct 06, 2020 |
Last update date |
Jan 20, 2022 |
Contact name |
Miao Xu |
Organization name |
West China hospital of Sichuan university
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Department |
Clinical Nutrition
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Street address |
37#,Guoxue alley, Wuhou district
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City |
Chengdu |
ZIP/Postal code |
610041 |
Country |
China |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (18)
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Relations |
BioProject |
PRJNA667723 |
SRA |
SRP286553 |