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Series GSE159120 Query DataSets for GSE159120
Status Public on Jun 28, 2021
Title RNA Sequencing of PGE2 or MEHP treated human THP-1 derived macrophages transcriptomes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary THP-1 cells were treated with phorbol 12-myristate 13-acetate (PMA) at 25μg/ml for 48h.Then remove PMA and rest for 24h to obtain M0 macropahges. Further applied LPS and IFNγ or IL-4 and IL-13 for 24h to obtain M1, M2 macrophages, respectively. During polarization, cells were treated with prostaglandin E2 or MEHP.Then M1,M2 cells were collected and applied to RNA-sequencing. We found that PGE2 and MEHP significantly impacted metabolic signature of macrophages.
 
Overall design mRNA profiles of PGE2 or MEHP treated M1 and M2 macrophages were examined by Illumina Hiseq.
 
Contributor(s) Yang H, Xu M
Citation(s) 34149684, 35019730
Submission date Oct 06, 2020
Last update date Jan 20, 2022
Contact name Miao Xu
Organization name West China hospital of Sichuan university
Department Clinical Nutrition
Street address 37#,Guoxue alley, Wuhou district
City Chengdu
ZIP/Postal code 610041
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (18)
GSM4819762 M1-CT-rep1
GSM4819763 M1-CT-rep2
GSM4819764 M1-CT-rep3
Relations
BioProject PRJNA667723
SRA SRP286553

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE159120_gene_fpkm-2.txt.gz 4.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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