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| Status |
Public on Jun 28, 2021 |
| Title |
RNA Sequencing of human THP-1 derived macrophages transcriptomes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
THP-1 cells were treated with phorbol 12-myristate 13-acetate (PMA) at 25μg/ml for 48h.Then remove PMA and rest for 24h to obtain M0 macropahges. Further applied LPS and IFNγ or IL-4 and IL-13 for 24h to obtain M1, M2 macrophages, respectively. During polarization, cells were growth within control medium(LN) or medium with extra lipid mixture(LH).M0,M1,M2 cells were collected and applied to RNA-sequencing. We found that differential activation of macrophage had distinct metabolic signature.Extra lipids supplement did not significantly alter transcriptomes of macrophages.
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| Overall design |
mRNA profiles of 3 different activation states of THP-1 derived macrophages within growth medium with/without extra lipid mixture were examined by Illumina Hiseq.
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| Contributor(s) |
Yang H, Xu M |
| Citation(s) |
34149684 |
| |
| Submission date |
Oct 06, 2020 |
| Last update date |
Jun 28, 2021 |
| Contact name |
Miao Xu |
| Organization name |
West China hospital of Sichuan university
|
| Department |
Clinical Nutrition
|
| Street address |
37#,Guoxue alley, Wuhou district
|
| City |
Chengdu |
| ZIP/Postal code |
610041 |
| Country |
China |
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| Platforms (1) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA667714 |
| SRA |
SRP286548 |
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