|
| Status |
Public on Mar 31, 2021 |
| Title |
Development of a glucocorticoid exposure signature for SLE |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed RNA-seq on peripheral blood mononuclear cells (PBMC) from healthy human donors treated with interferon alpha 2a (1000IU/mL) +/- dexamethasone 10^-7M, with the aims of studying interactions between IFN and glucocorticoid induced gene expression, as well as identifying potential transcripts that may be used as a glucocorticoid exposure signature in clinical practice in patients with SLE. Transcripts identified from RNA-seq data were analysed in SLE patient data sets to validate clinical utility. We found that dexamethasone minimally impacts on interferon stimulated gene expression however IFN altered gene transcription of many previously reported glucocorticoid induced genes.
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| |
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| Overall design |
Examination of IFN and dexamethasone induced gene expression changes in human PBMC.
|
| Web link |
https://doi.org/10.1016/S2665-9913(21)00006-0
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| |
|
| Contributor(s) |
Gearing LJ, Northcott M, Morand E |
| Citation |
Glucocorticoid gene signatures in systemic lupus erythematosus and the effects of type I interferon: a cross-sectional and in-vitro study
The Lancet Rheumatology Volume 3, Issue 5, May 2021, Pages e357-e370
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| |
| Submission date |
Oct 06, 2020 |
| Last update date |
Aug 03, 2021 |
| Contact name |
Jamie Gearing |
| Organization name |
Hudson Institute of Medical Research
|
| Street address |
27-31 Wright St
|
| City |
Melbourne |
| ZIP/Postal code |
3168 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA667695 |
| SRA |
SRP286533 |
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