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| Status |
Public on Oct 07, 2020 |
| Title |
Predicting the functional states of human iPSC-derived neurons with single-cell RNA-seq and electrophysiology |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Neural progenitors derived from human pluripotent stem cells (hPSCs) develop into electrophysiologically active neurons at heterogeneous rates, which can confound disease-relevant discoveries in neurology and psychiatry. The goal of this study was to resolve the diversity of functional states of hPSC-derived neurons in vitro by combining patch clamping with morphological and RNA-sequencing analysis of single neurons (Patch-seq).
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| Overall design |
Whole-transcriptome, single-cell mRNA-seq profiles of 56 patch-clamped and morphologically characterized cells (50 differentiated neurons and 6 astrocytes) generated using Smart-seq (SMARTer) chemistry.
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| Contributor(s) |
Bardy C, van den Hurk M, Gage FH |
| Citation(s) |
27698428 |
| |
| Submission date |
Oct 06, 2020 |
| Last update date |
Oct 12, 2020 |
| Contact name |
Cedric Bardy |
| Organization name |
South Australian Health and Medical Research Institute (SAHMRI)
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| Lab |
Laboratory for Human Neurophysiology and Genetics
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| Street address |
North Terrace
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| City |
Adelaide |
| State/province |
SA |
| ZIP/Postal code |
5000 |
| Country |
Australia |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (56)
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| Relations |
| BioProject |
PRJNA667623 |
| SRA |
SRP286496 |
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