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Series GSE159074 Query DataSets for GSE159074
Status Public on Oct 07, 2020
Title Predicting the functional states of human iPSC-derived neurons with single-cell RNA-seq and electrophysiology
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Neural progenitors derived from human pluripotent stem cells (hPSCs) develop into electrophysiologically active neurons at heterogeneous rates, which can confound disease-relevant discoveries in neurology and psychiatry. The goal of this study was to resolve the diversity of functional states of hPSC-derived neurons in vitro by combining patch clamping with morphological and RNA-sequencing analysis of single neurons (Patch-seq).
Overall design Whole-transcriptome, single-cell mRNA-seq profiles of 56 patch-clamped and morphologically characterized cells (50 differentiated neurons and 6 astrocytes) generated using Smart-seq (SMARTer) chemistry.
Contributor(s) Bardy C, van den Hurk M, Gage FH
Citation(s) 27698428
Submission date Oct 06, 2020
Last update date Oct 12, 2020
Contact name Cedric Bardy
Organization name South Australian Health and Medical Research Institute (SAHMRI)
Lab Laboratory for Human Neurophysiology and Genetics
Street address North Terrace
City Adelaide
State/province SA
ZIP/Postal code 5000
Country Australia
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (56)
GSM4818780 Cell #3
GSM4818781 Cell #4
GSM4818782 Cell #13
BioProject PRJNA667623
SRA SRP286496

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Supplementary file Size Download File type/resource
GSE159074_Bardy2016_expr_df_PatchSeq_56cells_TPM.txt.gz 2.2 Mb (ftp)(http) TXT
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Processed data are available on Series record

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