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| Status |
Public on Dec 15, 2021 |
| Title |
Transcriptome analysis using Quant-RNAseq after NPM1 or HIF-1α silencing under normoxia or Hypoxia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Hypoxia inducible factor-1 (HIF-1) is a central transcriptional regulator of genes associated with adaptive responses to hypoxia. NPM1 is a histone chaperone found to associate with HIF-1α in a phosphorylation dependent manner and increase its activty. The aim of this study was to find if HIF-1α and NPM1 regualate gene expression under hypoxia. Transcriptome analysis using Quant-RNA-seq after HIF-1α or NPM1 silencing under hypoxia reveals a significant number of genes, the hypoxic expression of which depends on both proteins.
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| Overall design |
Examinaton of genes expressed after silencing with siRNA NPM1 or HIF-1α in HeLa cells and subsequent incubaton in normaxia or hypoxia.
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| Contributor(s) |
Koukoulas K, Giakountis A, Simos G, Mylonis I |
| Citation(s) |
34388291 |
| |
| Submission date |
Oct 01, 2020 |
| Last update date |
Dec 15, 2021 |
| Contact name |
Antonis Giakountis |
| Organization name |
University of Thessaly
|
| Department |
Biochemistry and Biotechnology
|
| Lab |
Laboratory of Molecular Biology and Genomics
|
| Street address |
Biopolis
|
| City |
Larissa |
| ZIP/Postal code |
41500 |
| Country |
Greece |
| |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA666907 |
| SRA |
SRP286039 |
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