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| Status |
Public on Jan 18, 2021 |
| Title |
RNA sequencing of ADIPOR2 knockout HEK293 cells undergone various treatments with palmitic acid |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
How cells maintain vital membrane lipid homeostasis while obtaining most of their constituent fatty acids from a varied diet remains largely unknown. Here, we used transcriptomics, lipidomics, growth and respiration assays and membrane property analyses in human HEK293 cells or human umbilical vein endothelial cells (HUVEC) to show that the function of AdipoR2 is to respond to membrane rigidification by regulating many lipid metabolism genes. We also show that AdipoR2-dependent membrane homeostasis is critical for growth and respiration in cells challenged with saturated fatty acids. Additionally, we found that AdipoR2 deficiency causes transcriptome and cell physiological defects similar to those observed in SREBP-deficient cells upon SFA challenge. Finally, we compared several genes considered important for lipid homeostasis, namely AdipoR2, SCD, FADS2, PEMT and ACSL4, and found that AdipoR2 and SCD are the most important among these to prevent membrane rigidification and excess saturation when human cells are challenged with exogenous SFAs. We conclude that AdipoR2-dependent membrane homeostasis is especially important for the non-toxic uptake of SFAs.
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| Overall design |
Triplicate samples of knockout (KO) and wildtype (WT) cells treated with BSA (control) or Palmitic acid (PA) for 0, 3, 9 and 24h
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| |
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| Contributor(s) |
Palmgren H, Ruiz M, Pilon M |
| Citation(s) |
33444759 |
| |
| Submission date |
Sep 30, 2020 |
| Last update date |
Apr 19, 2021 |
| Contact name |
Marc Pilon |
| E-mail(s) |
marc.pilon@cmb.gu.se
|
| Organization name |
University of Gothenburg
|
| Department |
Chemistry and Molecular Biology
|
| Street address |
Medicinaregetan 9 (BOX462)
|
| City |
Gothenburg |
| ZIP/Postal code |
40530 |
| Country |
Sweden |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (42)
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| Relations |
| BioProject |
PRJNA666670 |
| SRA |
SRP285935 |
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