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Series GSE158769 Query DataSets for GSE158769
Status Public on May 24, 2021
Title Multimodal profiling of 500,000 memory T cells from a tuberculosis cohort
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We profiled peripheral memory T cells from donors with prior TB disease or latent infection to identify cell states associated with disease or other demographic and environmental variables. For higher-resolution definition of T cell states, we performed CITE-seq to measure single-cell mRNA and surface expression of 31 key memory T cell proteins, integrated these modalities, and quantified asssociations with variables of interest.
 
Overall design PBMCs were collected from 259 donors, including former progressors (cases) and non-progressors (controls). Technical replicates were included across separate batches for 12. We isolated memory T cells and profiled 500,089 high-quality cells with CITE-seq (using a surface marker panel of 31 antibodies). Raw sequencing data for human samples will be available on dbGAP.
 
Contributor(s) Nathan A, Raychaudhuri S
Citation(s) 34031617
Submission date Sep 29, 2020
Last update date Sep 03, 2021
Contact name Aparna Nathan
Organization name Harvard Medical School, Brigham and Women's Hospital
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (1)
GSM4810298 Memory_T_cell_CITE-seq
Relations
BioProject PRJNA666446

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Supplementary file Size Download File type/resource
GSE158769_exprs_norm.tsv.gz 3.0 Gb (ftp)(http) TSV
GSE158769_exprs_raw.tsv.gz 747.0 Mb (ftp)(http) TSV
GSE158769_meta_data.txt.gz 20.4 Mb (ftp)(http) TXT
Raw data not provided for this record
Processed data are available on Series record

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