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Series GSE158663 Query DataSets for GSE158663
Status Public on Apr 26, 2021
Title Hakai is required for stabilizing core components of the m6A mRNA methylation machinery
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary N6-methyladenosine RNA (m6A) is the most abundant internal modification on mRNA which influences most steps of mRNA metabolism and is involved in several biological functions, including circadian clock, metabolism and embryonic stem cell differentiation. The E3 ubiquitin ligase Hakai was previously found in complex with components of the m6A methylation machinery in plants and mammalian cells but its precise function remained to be investigated. Here we show that Hakai is a conserved component of the methyltransferase complex in Drosophila. Its depletion results in reduced m6A levels and altered m6A-dependent functions including sex determination. We show that its ubiquitination domain is required for dimerisation and interaction with other members of the m6A machinery, while its catalytic activity seems dispensable. Finally, we demonstrate that the loss of Hakai destabilizes the level of several subunits of the methyltransferase complex, resulting in impaired m6A deposition. Thus, our work adds new functional and molecular insights into the mechanism of the m6A mRNA writer complex.
Overall design S2R+ cells were treated with corresponding double stranded RNA three times during 6 days
Contributor(s) Lence T, Kreim N, Roignant J
Citation(s) 34145251
Submission date Sep 28, 2020
Last update date Jul 08, 2021
Contact name Jean-Yves Roignant
Organization name Institute of molecular Biology
Lab Roignant
Street address Ackermannweg 4
City Mainz
ZIP/Postal code 55128
Country Germany
Platforms (1)
GPL19132 Illumina NextSeq 500 (Drosophila melanogaster)
Samples (6)
GSM4805177 01_Ctr_rep1
GSM4805178 02_Ctr_rep2
GSM4805179 03_Ctr_rep3
BioProject PRJNA666156
SRA SRP285631

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Supplementary file Size Download File type/resource
GSE158663_RAW.tar 440.0 Kb (http)(custom) TAR (of TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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