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Series GSE158089 Query DataSets for GSE158089
Status Public on Jun 03, 2021
Title Characterisation of DNA methylomic signatures of induced pluripotent stem cells during neuronal differentiation
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary In development, inducing pluripotency and differentiation into different cell types results in global epigenetic changes, although the extent this occurs in induced pluripotent stem cell (iPSC)-based neuronal models has not been extensively characterized. We have differentiated iPSCs (33Qn1 cell line) into cortical neurons and assessed their DNA methylation profile at different time points during differentiation and maturation to identify DNA methylomic trajectories of neuronal aging. Samples were collected at four different time points, these were at the iPSC, neuronal precursor (NPC) and two different terminally differentiated neuronal time points (day 37 and day 58). We have identified loci undergoing significant DNA methylation changes throughout differentiation, have created an epigenetic trajectory signature associated with differentiation/maturation and identified highly connected and influential loci using gene-gene interaction analysis.
Overall design Samples were collected at four different time points during the differentiation and maturation of iPSCs into cortical neurons. Four replicates were collected for each time point apart from the iPSC stage where there are only two replicates.
Contributor(s) Imm J, Pishva E, Muhammed A, Kerrigan TL, Jeffries A, Burrage J, Glaab E, Cope EL, Jones KM, Allen ND, Lunnon K
Citation(s) 34277599
Submission date Sep 16, 2020
Last update date Jul 20, 2021
Contact name Jennifer Imm
Organization name University of Exeter
Department College of Medicine and Health
Lab RILD, RD&E Hospital
Street address Barrack Road
City Exeter
State/province Devon
ZIP/Postal code EX2 5DW
Country United Kingdom
Platforms (1)
GPL21145 Infinium MethylationEPIC
Samples (14)
GSM4790703 iPSC_1
GSM4790706 iPSC_2
GSM4790708 NPC_1
BioProject PRJNA663913

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE158089_RAW.tar 161.2 Mb (http)(custom) TAR
GSE158089_signal_intensities.csv.gz 102.4 Mb (ftp)(http) CSV
Raw data are available on Series record
Processed data included within Sample table

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