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| Status |
Public on Jul 05, 2022 |
| Title |
Single-cell multi-omics in human clonal hematopoiesis reveals that DNMT3A R882 mutations perturb early progenitor states through selective hypomethylation. |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Clonal hematopoiesis (CH) reflects clonal expansion of blood stem and progenitor cells with somatic mutations. We leveraged multi-modality single-cell sequencing to capture mutation status together with the transcriptome and methylome of CD34+ hematopoietic progenitor cells from five individuals with DNMT3A R882-mutated CH.
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| Overall design |
We profiled CD34+ enriched cells from GCSF mobilized bone marrow samples (n = 4) using single-cell RNA sequencing (10X) with targeted genotyping, and single-cell DNA methylation (RRBS) with single-cell RNA sequencing (Smart-Seq2) and targeted genotyping.
A 5th CH bone marrow aspirate sample was obtained to validate observed results.
**Submitter declares that the raw data are being deposited in EGA due to patient privacy concerns**
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| Contributor(s) |
Dusaj N, Nam A, Izzo F, Murali R, Chaligne R, Ghobrial I, Landau DA |
| Citation(s) |
36138229 |
| Submission date |
Sep 16, 2020 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Neville Dusaj |
| E-mail(s) |
ned2010@med.cornell.edu
|
| Organization name |
Weill Cornell Medicine
|
| Lab |
Landau Lab
|
| Street address |
413 E 69th St.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (3) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (26)
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| Relations |
| BioProject |
PRJNA663876 |
| SRA |
SRP282606 |
| SRA |
SRP376370 |
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