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Series GSE157852 Query DataSets for GSE157852
Status Public on Sep 23, 2020
Title Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Neurological complications are common in patients with COVID-19. While SARS-CoV-2, the causal pathogen of COVID-19, has been detected in some patient brains, its ability to infect brain cells and impact their function are not well understood, and experimental models using human brain cells are urgently needed. Here we investigated the susceptibility of human induced pluripotent stem cell (hiPSC)-derived monolayer brain cells and region-specific brain organoids to SARS-CoV-2 infection. We found modest numbers of infected neurons and astrocytes, but greater infection of choroid plexus epithelial cells. We optimized a protocol to generate choroid plexus organoids from hiPSCs, which revealed productive SARS-CoV-2 infection that leads to increased cell death and transcriptional dysregulation indicative of an inflammatory response and cellular function deficits. Together, our results provide evidence for SARS-CoV-2 neurotropism and support use of hiPSC-derived brain organoids as a platform to investigate the cellular susceptibility, disease mechanisms, and treatment strategies for SARS-CoV-2 infection.
 
Overall design Bulk RNA-seq of choroid plexus organoids (CPOs) was performed on mock 72 hours post-infection (hpi), SARS-CoV-2 24 hpi, and SARS-CoV-2 72 hpi samples. All conditions were profiled in triplicate.
 
Contributor(s) Pather SR
Citation(s) 33010822
Submission date Sep 11, 2020
Last update date Dec 23, 2020
Contact name Sarshan R. Pather
E-mail(s) sarshan.pather@pennmedicine.upenn.edu
Organization name University of Pennsylvania
Department Department of Neuroscience
Lab Clinical Research Building, Suite 105
Street address 415 Curie Boulevard
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (9)
GSM4776784 Mock 72 hpi rep1
GSM4776785 Mock 72 hpi rep2
GSM4776786 Mock 72 hpi rep3
Relations
BioProject PRJNA662965
SRA SRP282132

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE157852_CPO_RawCounts.txt.gz 416.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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