NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE157800 Query DataSets for GSE157800
Status Public on Sep 14, 2020
Title Loss of the seipin gene perturbs eggshell formation in Caenorhabditis elegans
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary SEIPIN, an evolutionary conserved protein, plays pivotal roles during lipid droplet (LD) biogenesis and is associated with various human diseases with unclear mechanisms. Here, we analyzed C. elegans mutants deleted of the sole SEIPIN gene, seip-1. Homozygous seip-1 mutants displayed penetrant embryonic lethality, which is caused by the disruption of the lipid-rich permeability barrier, the innermost layer of the C. elegans embryonic eggshell. In C. elegans oocytes and embryos, SEIP-1 is associated with LDs and crucial for controlling LD size and lipid homeostasis. The seip-1 deletion mutants reduced the ratio of polyunsaturated fatty acids (PUFAs) in their embryonic fatty acid pool. Interestingly, dietary supplementation of selected n-6 PUFAs rescued the embryonic lethality and defective permeability barrier. Accordingly, we propose that SEIP-1 may maternally regulate LD biogenesis and lipid homeostasis to orchestrate the formation of the permeability barrier for eggshell synthesis during embryogenesis. A lipodystrophy allele of seip-1 resulted in embryonic lethality as well and could be rescued by PUFA supplementation. These experiments support a great potential for using C. elegans to model seipin-associated human diseases.
 
Overall design Analysis of embryos and young adults representing seip-1_knock_outs.
 
Contributor(s) Chen S
Citation(s) 32820022
Submission date Sep 11, 2020
Last update date Feb 01, 2021
Contact name Leng-Jie Huang
E-mail(s) kkk12332141@gate.sinica.edu.tw
Organization name Academia Sinica
Department Institute of Plant and Microbial Biology
Street address 128 Sec. 2, Academia Rd, Nankang
City Taipei
ZIP/Postal code 11529
Country Taiwan
 
Platforms (1)
GPL11346 Agilent-020186 C. elegans (V2) Gene Expression Microarray (Probe Name version)
Samples (8)
GSM4775212 C.elegans_WT_embryo_rep1
GSM4775213 C.elegans_WT_embryo_rep2
GSM4775214 C.elegans_seip-1_KO_embryo_rep1
Relations
BioProject PRJNA663023

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE157800_RAW.tar 17.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap