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| Status |
Public on Mar 26, 2021 |
| Title |
The dynamic immune cell landscape in the lungs of Pneumocystis infected mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pneumocystis pneumonia is an opportunistic pneumonia that has been increasing in non-HIV patients in recent years. To obtain a better understanding of the cellular and molecular mechanisms involved in disease pathogenesis, we profile the transcriptomes of mouse lungs with Pneumocystis pneumonia and from uninfected control subjects using single-cell RNA sequencing, yielding multiple populations of myeloid cells, T cells and B cells. We uncover a PCP-associated TREM2+ subpopulation of interstitial macrophages, which expands in PCP, differentiates from Ly6C+ monocytes. We also define the subsets of effector CD4+ T cells that expand after the infection of Pneumocystis. Finally, intercellular crosstalk between interstitial macrophages and effector CD4+ T cells via multiple ligand and receptor interactions reveals several anti-pneumocystis pathways. Our work dissects unanticipated aspects of the cellular and molecular basis of Pneumocystis pneumonia at a single-cell level, and provides a conceptual framework for the discovery of rational therapeutic targets in Pneumocystis pneumonia.
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| |
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| Overall design |
Single-cell RNA profiles of wild type (WT) and mices infected with Pneumocystis 1-4 weeks.
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| Contributor(s) |
Tong Z, Zhai K, Yang H, Sun H |
| Citation(s) |
33959105, 38317180 |
| |
| Submission date |
Sep 08, 2020 |
| Last update date |
Feb 15, 2024 |
| Contact name |
Zhaohui Tong |
| E-mail(s) |
tongzhaohuicy@sina.com
|
| Phone |
13910930309
|
| Organization name |
Beijing Chaoyang Hospital
|
| Department |
Department of Respiratory and Critical Care Medicine
|
| Lab |
Beijing Institute of Respiratory Medicine
|
| Street address |
NO. 8, Gong Ti South Road
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100020 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA662263 |
| SRA |
SRP281194 |
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