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| Status |
Public on Feb 10, 2021 |
| Title |
MAPS integrates regulation of actin-targeting effector SteC into the virulence control network of Salmonella small RNA PinT |
| Organisms |
Mus musculus; Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study we take an integrative approach to capturing targets of the Hfq- associated sRNA PinT, a known post-transcriptional timer of the two major virulence programs of Salmonella enterica, using MS2 affinity purification and RNA- sequencing (MAPS).
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| Overall design |
MS2 affinity purification and RNA- sequencing (MAPS) to identify PinT ligands in bacteria under in-vitro conditions mimicking specific stages of the infection cycle, and in bacteria growing inside macrophages.
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| Contributor(s) |
Santos SC, Bischler T, Westermann AJ, Vogel J |
| Citation(s) |
33535041 |
| |
| Submission date |
Sep 04, 2020 |
| Last update date |
Feb 12, 2021 |
| Contact name |
Tom Gräfenhan |
| E-mail(s) |
ht-seq.sysmed@uni-wuerzburg.de
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| Phone |
+49 - 931 - 31 - 80814
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| Organization name |
University of Wuerzburg
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| Department |
Core Unit SysMed
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| Lab |
Raum D15.02.045
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| Street address |
Josef-Schneider-Str. 2, Bau D15
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| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
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| Platforms (2) |
| GPL20056 |
Illumina NextSeq 500 (Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344) |
| GPL20064 |
Illumina NextSeq 500 (Mus musculus; Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA661529 |
| SRA |
SRP280119 |
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