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| Status |
Public on Jun 02, 2009 |
| Title |
Chromosomal duplication in the grandifolia-D mutants of Arabidopsis thaliana, tiling array data |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome variation profiling by genome tiling array
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| Summary |
The number of cells in an organ is a major factor for the determination of organ size. However, genetic basis of cell number determination is not well understood. Three grandifolia-D (gra-D) mutants of Arabidopsis thaliana developed huge leaves containing two- to three-fold increased number of cells of the wild type. Tiling array and microarray analysis of gra-D mutants suggested that genes found in a lower part of chromosome 4 were upregulated, suggesting the occurrence of segmental chromosomal duplications in the gra-D mutants. These region contain positive regulators of cell proliferation such as AINTEGUMENTA (ANT) and cyclin genes such as CYCD3;1.
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| Overall design |
genomic DNA was extracted using DNeasy plant mini kit (Qiagen) from vegetative part of seedlings grown in vitro. DNA from wild type (wt, control) and the 3 grandifolia mutants (gra1-D, gra2-D and gra3-D) were submitted to tiling array hybridization.
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| Contributor(s) |
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H |
| Citation(s) |
19508432 |
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| Submission date |
Apr 17, 2009 |
| Last update date |
May 09, 2017 |
| Contact name |
Nathalie Gonzalez |
| E-mail(s) |
nagon@psb.vib-ugent.be
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| Phone |
+32 (0)9 331 39 56
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| Organization name |
VIB
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| Department |
Plant System Biology
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| Lab |
System Biology of Yield
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| Street address |
Technologiepark 927
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| City |
Gent |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
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| Platforms (1) |
| GPL10977 |
[At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE15726 |
Chromosomal duplication in the grandifolia-D mutants of Arabidopsis thaliana |
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| Relations |
| BioProject |
PRJNA122999 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15725_RAW.tar |
71.7 Mb |
(http)(custom) |
TAR (of BAR, BED, CEL) |
| Processed data provided as supplementary file |
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