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| Status |
Public on Feb 03, 2021 |
| Title |
alpha/beta/gamma/delta T cells play a vital role in fetal human skin development and immunity |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
T cells in adult human skin play a pivotal role in the defense against pathogens and tumors. While T cells can also be found in fetal human skin, little is known about their phenotype and function. Using single cell analyses, here we identify an unconventional naive T cell population expressing both alpha/beta and gamma/delta T cell receptors (TCRs) that was markedly enriched within early fetal skin and intestine but was untraceable in other fetal organs and peripheral blood. TCR sequencing data revealed that double-positive (DP) T cells displayed marginal or no overlap of CDR3 sequences with single-positive alpha/beta T cells. Gene signature, cytokine profile and in silico receptor-ligand interaction studies indicated that DP T cells contribute to skin development early in life. Finally, DP T cells were phosphoantigen-responsive suggesting that these cells participate in the protection of the fetus against pathogens in case of intrauterine infections. Together, our analyses unveil a unique and hitherto unrecognized cutaneous T cell type within the native skin microenvironment and point to fundamental differences in the adaptive immune surveillance of developing and adult human skin.
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| Overall design |
Three samples of human embryonic skin.
**Submitters declare that they can not provide raw sequencing data, as the data contain identifiable information.
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| Contributor(s) |
Reitermaier R, Krausgruber T, Fortelny N, Ayub T, Vieyra-Garcia P, Kienzl P, Wolf P, Scharrer A, Fiala C, Kölz M, Hiess M, Vierhapper M, Schuster C, Spittler A, Worda C, Weninger W, Bock C, Eppel W, Elbe-Bürger A |
| Citation(s) |
33561194, 34604909 |
| Submission date |
Aug 27, 2020 |
| Last update date |
Jan 27, 2022 |
| Contact name |
Christoph Bock |
| E-mail(s) |
cbock@cemm.oeaw.ac.at
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| Organization name |
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
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| Street address |
Lazarettgasse 14
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| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA659705 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE156972_CellAnnotation_AllCells.tsv.gz |
31.5 Kb |
(ftp)(http) |
TSV |
| GSE156972_CellAnnotation_Tcells.tsv.gz |
25.0 Kb |
(ftp)(http) |
TSV |
| GSE156972_raw_gene_bc_matrices_h5.h5 |
25.1 Mb |
(ftp)(http) |
H5 |
| Raw data not provided for this record |
| Processed data are available on Series record |
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