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| Status |
Public on Aug 30, 2021 |
| Title |
Transcriptome analysis of first-of-a-kind lines of conditionally immortalized human atrial cardiomyocytes (hiAMs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Using conditional immortalization, we generated the first lines of human cardiomyocytes with preserved cardiomyogenic differentiation capacity. These human immortalized Atrial Myocytes (hiAMs) display strict control over proliferation and differentiation, allowing massive (quadrillion-fold) expansion followed by differentiation towards fully functional (i.e. excitable and contractile) human atrial cardiomyocytes. Here, we characterized the transcriptome of three hiAM monoclones during proliferation (D0) and after differentiation (D12) using RNA-sequencing.
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| Overall design |
Total RNA of hiAM monoclones 2.38, 2.52 and 2.90 was sequenced during proliferation (D0) and after differentiation (D12). Per monoclone and time point, three replicates were used.
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| Contributor(s) |
Harlaar N, Pijnappels DA, de Vries AA |
| Citation missing |
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| |
| Submission date |
Aug 25, 2020 |
| Last update date |
Sep 02, 2021 |
| Contact name |
Antoine de Vries |
| E-mail(s) |
A.A.F.de_Vries@lumc.nl
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| Organization name |
Leiden University Medical Center
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| Department |
Cardiology
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| Lab |
Laboratory of Experimental Cardiology
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| Street address |
Albinusdreef 2
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| City |
Leiden |
| ZIP/Postal code |
2333ZA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA659284 |
| SRA |
SRP278819 |
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