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Series GSE156824 Query DataSets for GSE156824
Status Public on Aug 30, 2021
Title Transcriptome analysis of first-of-a-kind lines of conditionally immortalized human atrial cardiomyocytes (hiAMs)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Using conditional immortalization, we generated the first lines of human cardiomyocytes with preserved cardiomyogenic differentiation capacity. These human immortalized Atrial Myocytes (hiAMs) display strict control over proliferation and differentiation, allowing massive (quadrillion-fold) expansion followed by differentiation towards fully functional (i.e. excitable and contractile) human atrial cardiomyocytes. Here, we characterized the transcriptome of three hiAM monoclones during proliferation (D0) and after differentiation (D12) using RNA-sequencing.
 
Overall design Total RNA of hiAM monoclones 2.38, 2.52 and 2.90 was sequenced during proliferation (D0) and after differentiation (D12). Per monoclone and time point, three replicates were used.
 
Contributor(s) Harlaar N, Pijnappels DA, de Vries AA
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Submission date Aug 25, 2020
Last update date Sep 02, 2021
Contact name Antoine de Vries
E-mail(s) A.A.F.de_Vries@lumc.nl
Organization name Leiden University Medical Center
Department Cardiology
Lab Laboratory of Experimental Cardiology
Street address Albinusdreef 2
City Leiden
ZIP/Postal code 2333ZA
Country Netherlands
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (18)
GSM4745081 2.38-D0-1
GSM4745082 2.38-D0-2
GSM4745083 2.38-D0-3
Relations
BioProject PRJNA659284
SRA SRP278819

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156824_RAW.tar 32.9 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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