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| Status |
Public on Oct 28, 2020 |
| Title |
Single-cell RNAseq of CD45+ cells in murine gastric cancer. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
YTN2 and YTN16 were chemically induced gastric cancer cell lines. When inoculated subcutaneously, YTN2 spontaneously regresses while YTN16 grows progressively. By single-cell RNA-Seq and flow cytometry, we found more IL-17 producing cells in YTN16 than YTN2. Blockade of IL-17 signaling in YTN16 tumor-bearing mice suppressed tumor growth. To investigate tumor-infiltrating cells after IL-17 blockade, we performed single-cell RNA-Seq of CD45+ cells in YTN16 tumors.
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| Overall design |
Mice were inoculated with 5 ×106 YTN16 cells on day 0. Anti-IL-17 antibody (clone 17F3) was injected on days 0, 3, 6, and 9. On day 10, CD45+ cells were sorted from tumor tissues and processed by 10x controller with Single Cell 3' Reagent Kits v2.
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| Contributor(s) |
Nagaoka K, Maejima K, Fujita M, Nakagawa H, Kakimi K |
| Citation(s) |
33093158 |
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| Submission date |
Aug 24, 2020 |
| Last update date |
Oct 28, 2020 |
| Contact name |
Koji Nagaoka |
| Organization name |
Kindai University Faculty of Medicine
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| Department |
Department of Immunology
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| Street address |
377-2 Ohno-Higashi, Osaka-Sayama
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| City |
Osaka |
| ZIP/Postal code |
5898511 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA658905 |
| SRA |
SRP278586 |
