|
| Status |
Public on Feb 10, 2022 |
| Title |
ChIP-seq on Zfp961 GFP flox/flox mESCs to verify Zfp961 binding capacity at PBS-Lys elements. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We performed ChIP-seq assay with anti-GFP antibodies on Zfp961-GFPflox/flox ESCs.We found PBS-Lys-containing endogenous virus K subgroup repeats (ERV-K) were significantly enriched in the strong peak regions (40%, 165 out of 413), compared to their abundance in the mouse genome (~5%), suggesting Zfp961 binding preference towards ERV-K regions. And Zfp961 has a PBS-Lys motif. We performed H3K9me3 ChIP on Zfp961 WT or KO mESCs. We found that Zfp961 recruits H3k9me3 modification at ERVKs, and H3K9me3 level showed a moderate decrease upon Zfp961 deletion.
|
| |
|
| Overall design |
We used two replicates of each ChIP reaction. Basically, two Zfp961-GFP floxed mESCs cell lines treated with 4-OHT or etOH as control were used for anti-GFP ChIP and anti-H3K9me3 ChIP.
|
| |
|
| Contributor(s) |
Yang B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 21, 2020 |
| Last update date |
Nov 09, 2022 |
| Contact name |
Bo Yang |
| E-mail(s) |
yangbo2099@sina.cn
|
| Phone |
15221512158
|
| Organization name |
The Third Affiliated Hospital of Guangzhou Medical University
|
| Lab |
Lab of Sun
|
| Street address |
Guangzhou, 510150, Guangdong Province, China.
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510150 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE156604 |
Krab-ZFPs Inhibit Retrovirus by Targeting PBS Sites |
|
| Relations |
| BioProject |
PRJNA658492 |
| SRA |
SRP278351 |
Less...
More...