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Series GSE156540 Query DataSets for GSE156540
Status Public on Apr 15, 2021
Title Protease-mediated processing of Argonaute proteins controls small RNA association [small RNA RIP-seq]
Organism Caenorhabditis elegans
Experiment type Other
Summary Small RNA pathways defend the germlines of animals against selfish genetic elements and help to maintain genomic integrity. At the same time, their activity needs to be well-controlled to prevent silencing of ‘self’ genes. Here, we reveal a proteolytic mechanism that controls endogenous small interfering (22G) RNA activity in the Caenorhabditis elegans germline to protect genome integrity and maintain fertility. We find that WAGO-1 and WAGO-3 Argonaute proteins are produced as pro-proteins that are matured through proteolytic processing of their unusually proline-rich N-termini. In the absence of DPF-3, a P-granule-localized N-terminal dipeptidase orthologous to mammalian DPP8/9, processing fails, causing a change of identity of 22G RNAs bound to these WAGO proteins. Accumulation of repeat- and transposon-derived transcripts, DNA damage and sterility ensue. We propose that DPF-3 acts as a licensing factor for 22G RNA activity.
Overall design Checking the differential expression of small noncoding RNAs
Contributor(s) Grosshans H, Gudipatti RK, Gypas F
Citation(s) 33852894
Submission date Aug 20, 2020
Last update date Jul 15, 2021
Contact name Foivos Gypas
Organization name FMI
Lab Grosshans
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL18245 Illumina HiSeq 2500 (Caenorhabditis elegans)
Samples (12)
GSM4733239 1xFLAG::CSR-1. Replicate A. Input
GSM4733240 1xFLAG::CSR-1. Replicate B. Input
GSM4733241 1xFLAG::CSR-1. Replicate C. Input
This SubSeries is part of SuperSeries:
GSE151717 Protease-mediated processing of Argonaute proteins controls small RNA association
BioProject PRJNA658311
SRA SRP278193

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE156540_counts.tsv.gz 812.3 Kb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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