|
| Status |
Public on Apr 15, 2021 |
| Title |
Protease-mediated processing of Argonaute proteins controls small RNA association [small RNA RIP-seq] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Other
|
| Summary |
Small RNA pathways defend the germlines of animals against selfish genetic elements and help to maintain genomic integrity. At the same time, their activity needs to be well-controlled to prevent silencing of ‘self’ genes. Here, we reveal a proteolytic mechanism that controls endogenous small interfering (22G) RNA activity in the Caenorhabditis elegans germline to protect genome integrity and maintain fertility. We find that WAGO-1 and WAGO-3 Argonaute proteins are produced as pro-proteins that are matured through proteolytic processing of their unusually proline-rich N-termini. In the absence of DPF-3, a P-granule-localized N-terminal dipeptidase orthologous to mammalian DPP8/9, processing fails, causing a change of identity of 22G RNAs bound to these WAGO proteins. Accumulation of repeat- and transposon-derived transcripts, DNA damage and sterility ensue. We propose that DPF-3 acts as a licensing factor for 22G RNA activity.
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| |
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| Overall design |
Checking the differential expression of small noncoding RNAs
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| |
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| Contributor(s) |
Grosshans H, Gudipatti RK, Gypas F |
| Citation(s) |
33852894 |
| |
| Submission date |
Aug 20, 2020 |
| Last update date |
Jul 15, 2021 |
| Contact name |
Foivos Gypas |
| E-mail(s) |
foivos.gypas@fmi.ch
|
| Organization name |
FMI
|
| Lab |
Grosshans
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
|
| Samples (12)
|
| GSM4733242 |
WAGO-1::FLAG P3G, WAGO-3 P2G A3E. Replicate A. Input |
| GSM4733243 |
WAGO-1::FLAG P3G, WAGO-3 P2G A3E. Replicate B. Input |
| GSM4733244 |
WAGO-1::FLAG P3G, WAGO-3 P2G A3E. Replicate C. Input |
| GSM4733245 |
1xFLAG::CSR-1. Replicate A. IP |
| GSM4733246 |
1xFLAG::CSR-1. Replicate B. IP |
| GSM4733247 |
1xFLAG::CSR-1. Replicate C. IP |
| GSM4733248 |
WAGO-1::FLAG P3G, WAGO-3 P2G A3E. Replicate A. IP |
| GSM4733249 |
WAGO-1::FLAG P3G, WAGO-3 P2G A3E. Replicate B. IP |
| GSM4733250 |
WAGO-1::FLAG P3G, WAGO-3 P2G A3E. Replicate C. IP |
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| This SubSeries is part of SuperSeries: |
| GSE151717 |
Protease-mediated processing of Argonaute proteins controls small RNA association |
|
| Relations |
| BioProject |
PRJNA658311 |
| SRA |
SRP278193 |
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