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Status |
Public on Aug 31, 2021 |
Title |
Mechanisms of failing erythropoiesis in Diamond-Blackfan anemia revealed by single cell profiling of human bone marrow progenitors |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Single-cell RNAseq of CD34+Lineage negative hematopoietic stem and progenitor healthy control and DBA was performed to elucidate the transcriptional features of DBA haematopoiesis.
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Overall design |
Single cell transcriptional profiling using the 10x Genomics platform was performed on 45888 CD34+ Lineage- hematopoietic stem and progenitor cells FACS- isolated from 3 RPS-DBA samples, 3 RPL-DBA samples, and 3 healthy donor controls.
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Contributor(s) |
Iskander D, Wang G, Heuston EF |
Citation(s) |
34516827 |
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Submission date |
Aug 18, 2020 |
Last update date |
Nov 20, 2024 |
Contact name |
Jens Lichtenberg |
E-mail(s) |
lichtenbergj@mail.nih.gov
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Phone |
7402749495
|
Fax |
3014024929
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Organization name |
National Institutes of Health
|
Department |
National Human Genome Research Institute
|
Street address |
49 Convent Dr 3A19
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
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Platforms (2) |
GPL21697 |
NextSeq 550 (Homo sapiens) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (9)
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Relations |
BioProject |
PRJNA657889 |
SRA |
SRP277976 |